Specific detection of noradrenaline in the rat brain by using antibodies
| Autor: | Anne-Marie Rock, Josette Dulluc, Saraswati Patel, Michel Geffard |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Male
Immunocytochemistry Enzyme-Linked Immunosorbent Assay Immunoenzyme Techniques Norepinephrine chemistry.chemical_compound Antigen medicine Animals Molecular Biology Antiserum biology General Neuroscience Brain Molecular biology Dihydroxyphenylalanine In vitro Rats Substantia Nigra chemistry Biochemistry Antibody Formation Catecholamine biology.protein Locus Coeruleus Rabbits Neurology (clinical) Antibody Developmental Biology medicine.drug Conjugate |
| Zdroj: | Brain Research. 363:395-400 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(86)91031-0 |
| Popis: | Noradrenaline (NA) was conjugated to different protein carriers witglutaraldehyde. During the catecholamine conjugate synthesis, precautions were taken in order to preserve the structure of NA. Anti-NA antibodies were raised in rabbits and tested using a modified enzyme-linked immunosorbent assay and immunocytochemistry. The most immunoreactive antigen was found to be the reduced NA conjugate. The octopamine conjugate was recognized by the NA antiserum 16x less than the reduced NA conjugate. The other conjugates, dopamine, l -dihydroxyphenylalanine and adrenaline were poorly recognized by the anti-NA antibodies. A good correlation was found between results obtained using the in vitro enzyme-linked immunosorbent assay and immunocytochemistry. |
| Databáze: | OpenAIRE |
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