Age-related changes in sarcoplasmic reticulum Ca(2+)-ATPase and alpha-smooth muscle actin gene expression in aortas of normotensive and spontaneously hypertensive rats
| Autor: | F Lambert, T H Le Jemtel, G Gabbiani, D O Levitsky, M. Anger, M Clergue, A M Lompré |
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| Rok vydání: | 1993 |
| Předmět: |
Gene isoform
medicine.medical_specialty SERCA Physiology Gene Expression Aorta Thoracic Diaphragm pump Calcium-Transporting ATPases In Vitro Techniques Biology Rats Inbred WKY Muscle Smooth Vascular Rats Inbred SHR Internal medicine Gene expression medicine Animals RNA Messenger Actin Messenger RNA Endoplasmic reticulum Age Factors Blotting Northern Actins Rats Calcium ATPase Sarcoplasmic Reticulum Endocrinology Hypertension cardiovascular system Cardiology and Cardiovascular Medicine |
| Zdroj: | Circulation Research. 72:341-348 |
| ISSN: | 1524-4571 0009-7330 |
| DOI: | 10.1161/01.res.72.2.341 |
| Popis: | The expression of the sarcoplasmic reticulum (SR) Ca(2+)-ATPase gene and the SR Ca2+ pump function were investigated in thoracic aortas of 5- and 17-week-old normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHRs). The relative level of the two isoforms of SR Ca(2+)-ATPase mRNA expressed in the aorta (i.e., SERCA 2a and SERCA 2b) was determined by quantitative S1 nuclease protection analysis and normalized to the level of alpha-smooth muscle (alpha-Sm) actin mRNA. The level of alpha-Sm actin mRNA itself was normalized to the level of 18S ribosomal RNA using slot-blot hybridization assays. Total SR Ca2+ pump activity was estimated by measuring the rate of oxalate-supported Ca2+ uptake in homogenates. At 5 weeks, the amount of SERCA 2a and SERCA 2b mRNA, normalized to 18S ribosomal RNA, and the ratio of alpha-Sm actin mRNA to 18S RNA were identical in SHR and WKY rats. The Ca2+ pump activity was similar in the two strains of rats at 5 weeks. From 5 to 17 weeks, the amount of SERCA 2a mRNA increased in both strains while the level of SERCA 2b mRNA remained constant. The Ca2+ pump activity was unchanged in SHRs and tended to decrease in WKY rats. Accordingly, the change in the ratio of the SR Ca(2+)-ATPase mRNA isoforms does not appear to influence SR function. The level of alpha-Sm actin mRNA and SERCA 2a mRNA increased in parallel from 5 to 17 weeks in both strains.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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