Advantages of the scanning ion microscopy for mapping halogen corticoids in normal and transformed cells in culture
| Autor: | Danièle Chassoux, F. Escaig, Robert Dennebouy, George Slodzian, Jean-Michel Rossignol, P. Galle, Sylvie Tlouzeau, Marcel Chaintreau, Berry Jp |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Flumethasone
Nucleolus Scanning electron microscope Mineralogy chemistry.chemical_element Spectrometry Mass Secondary Ion Biology Triamcinolone Mice Structure-Activity Relationship Monolayer medicine Tumor Cells Cultured Animals Humans Glucocorticoids Cell Nucleus Cell Biology General Medicine 3T3 Cells Fluorine Cell nucleus medicine.anatomical_structure chemistry Fluocinolone Acetonide Cell culture Cancer cell Biophysics Microscopy Electron Scanning Field ion microscope Steroids Fluorinated |
| Zdroj: | Biology of the cell. 81(1) |
| ISSN: | 0248-4900 |
| Popis: | The intra-cellular distribution of eight halogen glucocorticoids was investigated by ion microscopy in two cellular varieties of cultured non-cancer cells (fibroblast 3T3) and cancer cells (human breast tumor cells MCF-7). Two types of ion microscopy helped to determine this distribution, a direct imaging ion microscope (SMI 300) with low spatial resolution, and a scanning ion microscope (IMS4F), featuring high resolution, serving to obtain maps representing the intra-cellular distribution of the fluorine elements and drugs present in these monolayer cultured cells. The fluorine images representative of the drugs containing fluorine showed that these drugs are essentially concentrated in the cell nuclei. In these nuclei, the distribution of these drugs is different from that of heterochromatin and of the nucleolus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text