Differential Chromatographic Bioassay for the Gentamicin Complex
| Autor: | Gerald H. Wagman, E. M. Oden, Marvin J. Weinstein |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Chromatography
Chloroform General Immunology and Microbiology Chromatography Paper Chemistry Staphylococcus Articles General Medicine medicine.disease_cause General Biochemistry Genetics and Molecular Biology Solvent Paper chromatography chemistry.chemical_compound Ammonium hydroxide Staphylococcus aureus Methods medicine Bioassay Biological Assay Gentamicin Methanol Gentamicins General Pharmacology Toxicology and Pharmaceutics medicine.drug |
| Zdroj: | Applied Microbiology. 16:624-627 |
| ISSN: | 0003-6919 |
| DOI: | 10.1128/am.16.4.624-627.1968 |
| Popis: | A differential paper chromatographic assay for the components of the gentamicin complex, designated as C 1 , C 1a , and C 2 , is described. The gentamicins are separated on paper strips with the lower phase of a solvent mixture consisting of chloroform, methanol, and 17% ammonium hydroxide (2: 1: 1, v/v). After chromatographic separation, the paper strips are plated against Staphylococcus aureus ATCC 6538P, and the zones of activity are quantified against known standards. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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