Local inhibition of matrix metalloproteinases reduced M2 macrophage activity and impeded recovery in spinal cord transected rats after treatment with fibroblast growth factor-1 and nerve grafts
Autor: | Wen Hung Huang, Huai Sheng Kuo, May Jywan Tsai, Meng-Jen Lee, Chuan Wen Chiu, Henrich Cheng, Ching Jung Chen |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
medicine.medical_specialty matrix metalloproteinase Population Central nervous system macrophage Fibroblast growth factor spinal cord injury fibroblast growth factor-1 GM6001 lcsh:RC346-429 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience Neurotrophic factors Internal medicine medicine education Spinal cord injury lcsh:Neurology. Diseases of the nervous system education.field_of_study Chemistry Spinal cord medicine.disease M2 Macrophage 030104 developmental biology medicine.anatomical_structure Endocrinology 030217 neurology & neurosurgery Research Article |
Zdroj: | Neural Regeneration Research Neural Regeneration Research, Vol 13, Iss 8, Pp 1447-1454 (2018) |
ISSN: | 1673-5374 |
Popis: | Alternatively activated macrophages (M2 macrophages) promote central nervous system regeneration. Our previous study demonstrated that treatment with peripheral nerve grafts and fibroblast growth factor-1 recruited more M2 macrophages and improved partial functional recovery in spinal cord transected rats. The migration of macrophages is matrix metalloproteinase (MMP) dependent. We used a general inhibitor of MMPs to influence macrophage migration, and we examined the migration of macrophage populations and changes in spinal function. Rat spinal cords were completely transected at T8, and 5 mm of spinal cord was removed (group T). In group R, spinal cord-transected rats received treatment with fibroblast growth factor-1 and peripheral nerve grafts. In group RG, rats received the same treatment as group R with the addition of 200 μM GM6001 (an MMP inhibitor) to the fibrin mix. We found that MMP-9, but not MMP-2, was upregulated in the graft area of rats in group R. Local application of the MMP inhibitor resulted in a reduction in the ratio of arginase-1 (M2 macrophage subset)/inducible nitric oxide synthase-postive cells. When the MMP inhibitor was applied at 8 weeks postoperation, the partial functional recovery observed in group R was lost. This effect was accompanied by a decrease in brain-derived neurotrophic factor levels in the nerve graft. These results suggested that the arginase-1 positive population in spinal cord transected rats is a migratory cell population rather than the phenotypic conversion of early iNOS+ cells and that the migration of the arginase-1+ population could be regulated locally. Simultaneous application of MMP inhibitors or promotion of MMP activity for spinal cord injury needs to be considered if the coadministered treatment involves M2 recruitment. |
Databáze: | OpenAIRE |
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