Transcriptome-wide analysis of the MADS-box gene family in the orchid Erycina pusilla
| Autor: | Wan-Jung Chang, Yao-Ting Huang, Ming-Lun Chou, Chen-Tran Hsu, De-Chih Liao, Jeremy J.W. Chen, Ming-Che Shih, Ming-Tsair Chan, Choun-Sea Lin, Swee-Suak Ko |
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| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Amino Acid Motifs Protein domain Arabidopsis MADS Domain Proteins Flowers Plant Science Biology Genes Plant 01 natural sciences Erycina pusilla Transcriptome 03 medical and health sciences Protein Domains Gene Expression Regulation Plant Databases Genetic Gene family Amino Acid Sequence Nucleotide Motifs Orchidaceae Gene Phylogeny MADS-box Genetics Phylogenetic tree Gene Expression Profiling fungi Intron food and beverages Exons Introns 030104 developmental biology Organ Specificity Multigene Family Agronomy and Crop Science Subcellular Fractions 010606 plant biology & botany Biotechnology |
| Zdroj: | Plant Biotechnology Journal. 14:284-298 |
| ISSN: | 1467-7644 |
| DOI: | 10.1111/pbi.12383 |
| Popis: | Orchids exhibit a range of unique flower shapes and are a valuable ornamental crop. MADS-box transcription factors are key regulatory components in flower initiation and development. Changing the flower shape and flowering time can increase the value of the orchid in the ornamental horticulture industry. In this study, 28 MADS-box genes were identified from the transcriptome database of the model orchid Erycina pusilla. The full-length genomic sequences of these MADS-box genes were obtained from BAC clones. Of these, 27 were MIKC-type EpMADS (two truncated forms) and one was a type I EpMADS. Eleven EpMADS genes contained introns longer than 10 kb. Phylogenetic analysis classified the 24 MIKC(c) genes into nine subfamilies. Three specific protein motifs, AG, FUL and SVP, were identified and used to classify three subfamilies. The expression profile of each EpMADS gene correlated with its putative function. The phylogenetic analysis was highly correlated with the protein domain identification and gene expression results. Spatial expression of EpMADS6, EpMADS12 and EpMADS15 was strongly detected in the inflorescence meristem, floral bud and seed via in situ hybridization. The subcellular localization of the 28 EpMADS proteins was also investigated. Although EpMADS27 lacks a complete MADS-box domain, EpMADS27-YFP was localized in the nucleus. This characterization of the orchid MADS-box family genes provides useful information for both orchid breeding and studies of flowering and evolution. |
| Databáze: | OpenAIRE |
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