An experimental vaccine cocktail for Plasmodium falciparum malaria
Autor: | Philip J. Barr, Benjamin K. Hahm, Isabella A. Quakyi, Wasim A. Siddiqui, Joe Kansopon, Ian C. Bathurst, Pascal Millet, Sandra P. Chang, Helen L. Gibson, Joseph Inselburg, David C. Kaslow, Kim M. Green, George Hui |
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Rok vydání: | 1993 |
Předmět: |
Protozoan Vaccines
medicine.medical_treatment Plasmodium falciparum Protozoan Proteins Antibodies Protozoan Antigens Protozoan Saccharomyces cerevisiae In Vitro Techniques Mice Immune system Antigen parasitic diseases medicine Animals Humans Cloning Molecular Malaria Falciparum Vaccines Synthetic General Veterinary General Immunology and Microbiology biology Immunogenicity Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Virology Recombinant Proteins Infectious Diseases Humoral immunity biology.protein Molecular Medicine Female Antibody Adjuvant Malaria |
Zdroj: | Vaccine. 11:449-456 |
ISSN: | 0264-410X |
Popis: | Surface proteins from several different life-cycle stages of the malaria parasite Plasmodium falciparum were expressed at high levels in the yeast Saccharomyces cerevisiae. Purified proteins, both individually and in cocktails, were used to immunize mice and goats in conjunction with either Freund's adjuvant or a muramyl tripeptide-based adjuvant. Immune responses were measured by enzyme-linked immunosorbent assays and by the ability of antisera to inhibit (1) the invasion of hepatocytes by live sporozoites, (2) in vitro invasion of human erythrocytes by live merozoites, and (3) the development of oocytes in the mosquito vector. These results suggest that cocktails of different stage-specific antigens can provide the components necessary to block the development of the malaria parasite at multiple stages of its life cycle. |
Databáze: | OpenAIRE |
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