Type 2 diabetes mellitus-associated transcriptome alterations in cortical neurones and associated neurovascular unit cells in the ageing brain
| Autor: | Bury, Joanna J, Chambers, Annabelle, Heath, Paul R, Ince, Paul G, Shaw, Pamela J, Matthews, Fiona E, Brayne, Carol, Simpson, Julie E, Wharton, Stephen B, Cognitive Function And Ageing Study |
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| Přispěvatelé: | Wharton, Stephen B [0000-0003-2785-333X], Apollo - University of Cambridge Repository, Wharton, Stephen B. [0000-0003-2785-333X] |
| Rok vydání: | 2021 |
| Předmět: |
Male
Candidate gene Aging medicine.medical_treatment Laser Capture Microdissection Biology lcsh:RC346-429 Pathology and Forensic Medicine Respiratory electron transport chain Transcriptome Cellular and Molecular Neuroscience Cortical neurone Differential expression Type 2 diabetes mellitus medicine Humans RNA Messenger lcsh:Neurology. Diseases of the nervous system Aged Temporal cortex Aged 80 and over Neurons Microarray analysis techniques Research Insulin Brain Endothelial Cells Temporal Lobe Cell biology Ageing medicine.anatomical_structure Diabetes Mellitus Type 2 Astrocytes Neurovascular unit Dementia Female Neurology (clinical) Astrocyte |
| Zdroj: | Acta Neuropathologica Communications Acta Neuropathologica Communications, Vol 9, Iss 1, Pp 1-16 (2021) |
| DOI: | 10.17863/cam.63905 |
| Popis: | Type 2 diabetes mellitus (T2D), characterised by peripheral insulin resistance, is a risk factor for dementia. In addition to its contribution to small and large vessel disease, T2D may directly damage cells of the brain neurovascular unit. In this study, we investigated the transcriptomic changes in cortical neurones, and associated astrocytes and endothelial cells of the neurovascular unit, in the ageing brain. Neurone, astrocyte, and endothelial cell-enriched mRNA, obtained by immuno-laser capture microdissection of temporal cortex (Brodmann area 21/22) from 6 cases with self-reported T2D in the Cognitive Function and Ageing Study neuropathology cohort, and an equal number of age and sex-matched controls, was assessed by microarray analysis. Integrated Molecular Pathway Level Analysis was performed using the Kyoto Encyclopaedia of Genes and Genomes database on significantly differentially expressed genes, defined as P |
| Databáze: | OpenAIRE |
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