Red wine extract prevents neuronal apoptosis in vitro and reduces mortality of transgenic mice
| Autor: | Rosalba Amodio, Caterina De Ruvo, Ennio Esposito, Giuseppe Carruba, Vincenzo Bellavia, Emanuele Amodio |
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| Přispěvatelé: | Amodio, R, Esposito, E, De Ruvo, C, Bellavia, V, Amodio, E, Carruba, G |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
SOD1
Glutamic Acid Apoptosis Mice Transgenic Wine Pharmacology Biology cerebellar granule cells • apoptosis • lyophilized red wine • ASL • mSOD1G93A General Biochemistry Genetics and Molecular Biology Mice Superoxide Dismutase-1 History and Philosophy of Science In vivo Animals Humans Cells Cultured chemistry.chemical_classification Neurons Reactive oxygen species Caspase 3 Superoxide Dismutase General Neuroscience Cytochrome c Cytochromes c Ascorbic acid Survival Analysis Neuroprotective Agents chemistry Biochemistry biology.protein Calcium Reactive Oxygen Species Intracellular |
| Popis: | In this work, we have investigated the effects of nutritional antioxidants as antidegenerative agents on glutamate-induced apoptosis in primary cultures of cerebellar granule neurons (CGNs). Glutamate-induced apoptosis is also associated with intracellular [Ca(2+)]i overload, generation of reactive oxygen species (ROS), depression of cell energy metabolism, cytochrome c release, and increase in caspase-3 activity. Pretreatment (3 h) with red wine extract (5 microg/mL) and ascorbic acid (30 microM) blocks glutamate-induced apoptosis in CGNs. In vivo experiments carried out on transgenic mice expressing the human mutated Cu, Zn superoxide dismutase (SOD1) G93A (mSOD1(G93A)) show that mice fed with lyophilized red wine have significantly increased survival as compared to control, untreated animals. |
| Databáze: | OpenAIRE |
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