Carbon metabolism in leaves of transgenic tobacco (Nicotiana tabacum L.) containing elevated fructose 2,6-bisphosphate levels
| Autor: | Peter Scott, Simon J. Pilkis, Alex J. Lange, Nicholas J. Kruger |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Sucrose
Phosphofructokinase-2 Nicotiana tabacum Metabolite Molecular Sequence Data Fructose 1 6-bisphosphatase Plant Science Carbohydrate metabolism Genes Plant chemistry.chemical_compound Tobacco Genetics Fructosediphosphates Animals DNA Primers biology Base Sequence Fructose Cell Biology biology.organism_classification Plants Genetically Modified Carbon Rats Phosphotransferases (Alcohol Group Acceptor) Plants Toxic chemistry Biochemistry Fructose 2 6-bisphosphate Liver biology.protein Carbohydrate Metabolism Solanaceae |
| Popis: | Summary The aim of this work was to investigate the role of fructose 2,6-bisphosphate (Fru 2,6-P2) during photosynthesis. The level of Fru 2,6-P2 in tobacco plants was elevated by the introduction of a modified mammalian gene encoding 6-phosphofructo-2-kinase (6-PF-2-K). Estimates of the metabolite control coefficient (C) for Fru 2,6-P2 levels in response to increased 6-PF-2-K activity, suggest that small increases in 6-PF-2-K activity have little effect upon steady-state Fru 2,6-P2 levels (C = +0.08 for a 0–58% increase in 6-PF-2-K activity). However, larger changes resulted in dramatic rises in Fru 2,6-P2 levels (C = +3.35 for 206–268% increase in 6-PF-2-K activity). Transgenic plants contained Fru 2,6-P2 levels in the dark that ranged from 104 to 230% of the level in wild-type tobacco. Plants with altered levels of Fru 2,6-P2 were used to determine the effects of this signal metabolite upon carbohydrate metabolism during the initial phase of the light period. Here we provide direct evidence that Fru 2,6-P2 contributes to the regulation of carbon partitioning in tobacco leaves by inhibiting sucrose synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|