Lysis of the zona pellucida and attachment of embryos to the uterine epithelium in ovariectomized mice treated with oestradiol-17 and progesterone
| Autor: | R. C. Hoversland, H. M. Weitlauf |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
endocrine system
Embryology medicine.medical_specialty Lysis Uterine epithelium Mice Endocrinology Internal medicine medicine Animals Castration Embryo Implantation Zona pellucida Uterine lumen Progesterone Zona Pellucida reproductive and urinary physiology Ovum Estradiol biology urogenital system Obstetrics and Gynecology Embryo Cell Biology Enzyme assay Blastocyst medicine.anatomical_structure Reproductive Medicine embryonic structures biology.protein Ovariectomized rat Female Oestradiol 17β hormones hormone substitutes and hormone antagonists |
| Zdroj: | Reproduction. 62:111-116 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/jrf.0.0620111 |
| Popis: | Summary. Preimplantation mouse embryos with intact zonae pellucidae were transferred into the uteri of ovariectomized females treated with progesterone, oestradiol-17\g=b\ plus progesterone, or oestradiol-17\g=b\alone; the disappearance of zonae from the uterine lumen was used to determine the presence of 'zona-lytic' activity in situ. Lysis of zonae did not occur in animals treated with progesterone or oestradiol-17\g=b\alone. However, lysis did occur when oestradiol-17 \g=b\was combined with progesterone; 'zona-lytic' activity reached peak levels within 12 to 24 h, then decreased. Attachment of embryos to the uterine epithelium occurred only in animals treated with oestradiol-17\g=b\plus progesterone and was initiated at about the time of peak 'zona-lytic' activity. It is suggested that the chymotrypsin-like enzyme activity present in uterine fluid is involved with the initiation of implantation. |
| Databáze: | OpenAIRE |
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