Poly(ε-Caprolactone) Nanofiber Wrap Improves Nerve Regeneration and Functional Outcomes after Delayed Nerve Repair
| Autor: | Sinan Xiang, Joshua Budihardjo, Leila Musavi, Gerald Brandacher, Zuhaib Ibrahim, Richard J. Redett, Angelo A. Leto Barone, Sara Mulla, Russell Martin, Amy Quan, Kevin Z. Xin, Hai-Quan Mao, W. P. Andrew Lee, Joseph Lopez |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Median Neuropathy Polyesters Nerve guidance conduit Nanofibers 030230 surgery 03 medical and health sciences Grip strength 0302 clinical medicine medicine Animals Axon Denervation business.industry Regeneration (biology) Recovery of Function Median nerve Compound muscle action potential Nerve Regeneration Rats Disease Models Animal medicine.anatomical_structure 030220 oncology & carcinogenesis Anesthesia Chronic Disease Surgery Forelimb business |
| Zdroj: | Plastic and reconstructive surgery. 144(1) |
| ISSN: | 1529-4242 |
| Popis: | BACKGROUND The purpose of this study was to assess the efficacy of biodegradable, electrospun poly(e-caprolactone) nanofiber nerve conduits in improving nerve regeneration. METHODS The authors used a rat forelimb chronic denervation model to assess the effects of poly(e-caprolactone) conduits on improving nerve regeneration and upper extremity function. Three groups of rats were examined: (1) negative-control animals (n = 5), which underwent 8 weeks of median nerve chronic denervation injury followed by repair with no conduit; (2) experimental animals (n = 5), which underwent 8 weeks of median nerve chronic denervation followed by repair and poly(e-caprolactone) nerve conduit wrapping of the nerve coaptation site; and (3) positive-control animals (n = 5), which were naive controls. All animals underwent compound muscle action potential and functional testing. At 14 weeks after repair, the median nerve and flexor muscles were harvested for histologic analysis. RESULTS Histomorphometric analysis of regenerating median nerves demonstrated augmented axonal regeneration in experimental versus negative control animals (total axon count, 1769 ± 672 versus 1072 ± 123.80; p = 0.0468). With regard to functional recovery, experimental and negative-control animals (1.67 ± 0.04 versus 0.97 ± 0.39; p = 0.036) had regained 34.9 percent and 25.4 percent, respectively, of baseline hand grip strength at 14 weeks after repair. Lastly, less collagen deposition at the nerve coaptation site of experimental animals was found when compared to control animals (p < 0.05). CONCLUSION Biodegradable, poly(e-caprolactone) nanofiber nerve conduits can improve nerve regeneration and subsequent physiologic extremity function in the setting of delayed nerve repair by decreasing the scar burden at nerve coaptation sites. |
| Databáze: | OpenAIRE |
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