Conformational Selection in the Recognition of Phosphorylated Substrates by the Catalytic Domain of Human Pin1
| Autor: | Donald Hamelberg, Hector A. Velazquez |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Protein Conformation
Peptide Molecular Dynamics Simulation Biology Crystallography X-Ray Biochemistry Substrate Specificity Serine Protein structure Catalytic Domain Humans Phosphorylation NIMA-Interacting Peptidylprolyl Isomerase Threonine chemistry.chemical_classification Drug Synergism Stereoisomerism Peptidylprolyl Isomerase Enzyme chemistry Drug Design PIN1 Protein Processing Post-Translational |
| Zdroj: | Biochemistry. 50:9605-9615 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi2009954 |
| Popis: | Post-translational phosphorylation and the related conformational changes in signaling proteins are responsible for regulating a wide range of subcellular processes. Human Pin1 is central to many of these cell signaling pathways in normal and aberrant subcellular processes, catalyzing cis-trans isomerization of the peptide ω-bond in phosphorylated serine/threonine-proline motifs in many proteins. Pin1 has therefore been identified as a possible drug target in many diseases, including cancer and Alzheimer's. The effects of phosphorylation on Pin1 substrates, and the atomistic basis for Pin1 recognition and catalysis, are not well understood. Here, we determine the conformational consequences of phosphorylation on Pin1 substrate analogues and the mechanism of recognition by the catalytic domain of Pin1 using all-atom molecular dynamics simulations. We show that phosphorylation induces backbone conformational changes on the peptide substrate analogues. We also show that Pin1 recognizes specific conformations of its substrate by conformational selection. Furthermore, dynamical correlated motions in the free Pin1 enzyme are present in the enzyme of the enzyme-substrate complex when the substrate is in the transition state configuration, suggesting that these motions play significant roles during catalytic turnover. These results provide a detailed atomistic picture of the mechanism of Pin1 recognition that can be exploited for drug design purposes and further our understanding of the synergistic complexities of post-translational phosphorylation and cis-trans isomerization. |
| Databáze: | OpenAIRE |
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