Evaluation of buccal damage associated with acute inhalation exposure to 2,4-dichlorophenoxyacetic acid (2,4-D) in mice
| Autor: | Fabíola de Azevedo Mello, Renata Calciolari Rossi, Ana Carolina Gomes Lisboa, Bruna Barravieira, Gisele Alborghetti Nai, Amanda Jodas Tolardo, José Luiz Santos Parizi |
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| Rok vydání: | 2020 |
| Předmět: |
Male
2 4-Dichlorophenoxyacetic acid Physiology 010501 environmental sciences 01 natural sciences Epithelium Pesticide exposure 03 medical and health sciences chemistry.chemical_compound Mice oral 0302 clinical medicine Tongue medicine Nucleolus Organizer Region Animals Oral mucosa 0105 earth and related environmental sciences Inhalation exposure Inflammation Inhalation Exposure lcsh:Veterinary medicine General Veterinary business.industry Herbicides Degranulation Mouth Mucosa 030206 dentistry General Medicine Buccal administration Environmental exposure Mast cell medicine.anatomical_structure chemistry Mast cells lcsh:SF600-1100 2 4-Dichlorophenoxyacetic Acid business Leukoplakia Research Article |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 16, Iss 1, Pp 1-10 (2020) |
| ISSN: | 1746-6148 |
| Popis: | Background: The herbicide dichlorophenoxyacetic acid (2,4-D) is one of the most widely used crop spraying products in the world. Some pesticides induce the degranulation of mast cells and increase allergic responses. This is the first study to evaluate the damage to the oral mucosa after an experimental simulation of environmental inhalation exposure to the 2,4-D herbicide. The aim of this study was evaluate the possible oral damage caused by acute inhalation exposure to the herbicide 2,4-D.Methods: A total of 80 Swiss male mice were divided into 4 groups (n = 20) and evaluated at different time intervals (24 h, 48 h, 72 h and 8 days), with 5 animals / period. The animals were divided into the following groups: SG: nebulization with sodium chloride solution; LCG: pesticide nebulization with 3.71 x 10-3 grams of active ingredient per hectare (g.i.a. / ha); MCG: pesticide nebulization with 6.19 x 10-3 g.i.a. /ha; and HCG: pesticide nebulization with 9.28 x 10-3 g.i.a. /ha. The tongues of the mice were collected for histopathological analysis.Results: There was a difference between the exposure concentrations in relation to tissue congestion intensity (p = 0.002) and mast cell counts (p = 0.002), a difference in the evaluation of the interaction between the exposure concentrations and nebulization time in the dorsum epithelium thickness (p = 0.013), and a significant correlation between the epithelial thickness and the number of nucleoli organizing regions on the dorsum of the tongue (p = 0.048).Conclusions: Even after acute exposure, the herbicide 2,4-D had the potential to damage the oral epithelium, especially at higher doses. |
| Databáze: | OpenAIRE |
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