Quantitative imaging of intraerythrocytic hemozoin by transient absorption microscopy
| Autor: | Robert L. Summers, Yimin Huang, Pu-Ting Dong, Kai-Chih Huang, Ji-Xin Cheng, Andy J. Chen, Dyann F. Wirth, Selina Bopp, Cheng Zong |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Chemical imaging
Paper Hemeproteins Quantitative imaging Erythrocytes Optical Phenomena Plasmodium falciparum Biomedical Engineering malaria Drug Evaluation Preclinical 01 natural sciences 010309 optics Biomaterials chemistry.chemical_compound transient absorption microscopy Antimalarials Treatment targets Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences hemozoin 0103 physical sciences Microscopy parasitic diseases Animals Humans chemical imaging Malaria Falciparum Heme Hemozoin Atomic and Molecular Physics and Optics 3. Good health Electronic Optical and Magnetic Materials High-Throughput Screening Assays chemistry Biophysics Microscopy Electron Scanning Single-Cell Analysis Crystallization label-free imaging |
| Zdroj: | Journal of Biomedical Optics |
| ISSN: | 1560-2281 1083-3668 |
| Popis: | Hemozoin, the heme detoxification end product in malaria parasites during their growth in the red blood cells (RBCs), serves as an important marker for diagnosis and treatment target of malaria disease. However, the current method for hemozoin-targeted drug screening mainly relies on in-vitro β-hematin inhibition assays, which may lead to false-positive events due to under-representation of the real hemozoin crystal. Quantitative in-situ imaging of hemozoin is highly desired for high-throughput screening of antimalarial drugs and for elucidating the mechanisms of antimalarial drugs. We present transient absorption (TA) imaging as a high-speed single-cell analysis platform with chemical selectivity to hemozoin. We first demonstrated that TA microscopy is able to identify β-hematin, the artificial form of hemozoin, from the RBCs. We further utilized time-resolved TA imaging to in situ discern hemozoin from malaria-infected RBCs with optimized imaging conditions. Finally, we quantitatively analyzed the hemozoin amount in RBCs at different infection stages by single-shot TA imaging. These results highlight the potential of TA imaging for efficient antimalarial drug screening and drug mechanism investigation. |
| Databáze: | OpenAIRE |
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