Metabolic engineering a yeast to produce astaxanthin
| Autor: | Yi-Ying Kao, Wen-Hsiung Li, Caroline Thia, Chieh-Chen Huang, Yu-Ju Lin, Jui-Jen Chang, Hao-Yeh Lin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Environmental Engineering Saccharomyces cerevisiae Mutagenesis (molecular biology technique) Bioengineering Xanthophylls Protein degradation Metabolic engineering 03 medical and health sciences chemistry.chemical_compound Kluyveromyces marxianus Chlorophyta Astaxanthin Waste Management and Disposal Haematococcus pluvialis biology Renewable Energy Sustainability and the Environment General Medicine biology.organism_classification Yeast 030104 developmental biology Metabolic Engineering chemistry Biochemistry Oxygenases |
| Zdroj: | Bioresource Technology. 245:899-905 |
| ISSN: | 0960-8524 |
| Popis: | In this study, an astaxanthin-biosynthesis Kluyveromyces marxianus strain Sm23 was first constructed, which could produce 31µg/g DCW astaxanthin. Then, repeated genome integration of the key astaxanthin biosynthesis genes Hpchyb and bkt was done to increase gene copy number and astaxanthin yield. Four improved strains were obtained and the yield of astaxanthin and the total yield of carotenoids in a strain increased with the copy numbers of Hpchyb and bkt. To improve the yield further, the gene Hpchyb from Haematococcus pluvialis was modified by site-directed mutagenesis to increase the enzyme efficiency or/and to prevent the heterologous protein degradation by ubiquitination. Using repeated-integration approach of bkt and the mutated Hpchyb into Sm23, the S3-2 strain was obtained and shown to produce the 3S, 3'S-astaxanthin at 9972µg/g DCW in a 5L fermentor. |
| Databáze: | OpenAIRE |
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