Transient receptor potential vanilloid type 1 is expressed in the horizontal pathway of the vervet monkey retina
| Autor: | Jean-François Bouchard, Catarina Micaelo-Fernandes, Joseph Bouskila, Maurice Ptito, Roberta M. Palmour |
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| Rok vydání: | 2020 |
| Předmět: |
Retinal Ganglion Cells
Cannabinoid receptor TRPV1 TRPV Cation Channels lcsh:Medicine Article Retina Chlorocebus aethiops medicine Animals Photoreceptor Cells Tissue Distribution Vervet monkey lcsh:Science Cell body membrane Transcription Factor Brn-3A Multidisciplinary biology Qa-SNARE Proteins Chemistry musculoskeletal neural and ocular physiology lcsh:R Inner plexiform layer biology.organism_classification Endocannabinoid system Cell biology Amacrine Cells Parvalbumins medicine.anatomical_structure nervous system Retinal ganglion cell Synapses lcsh:Q lipids (amino acids peptides and proteins) sense organs psychological phenomena and processes |
| Zdroj: | Bouskila, J, Micaelo-Fernandes, C, Palmour, R M, Bouchard, J F & Ptito, M 2020, ' Transient receptor potential vanilloid type 1 is expressed in the horizontal pathway of the vervet monkey retina ', Scientific Reports, vol. 10, 12116 . https://doi.org/10.1038/s41598-020-68937-9 Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-68937-9 |
| Popis: | The ubiquitous distribution of the classic endocannabinoid system (cannabinoid receptors CB1 and CB2) has been demonstrated within the monkey nervous system, including the retina. Transient receptor potential vanilloid type 1 (TRPV1) is a cannabinoid-like non-selective cation channel receptor that is present in the retina and binds to endovannilloids and endocannabinoids, like anandamide, 2-arachidonoylglycerol and N-arachidonoyl dopamine. Retinal expression patterns of TRPV1 are available for rodents and data in higher mammals like humans and monkeys are scarce. We therefore thoroughly examined the expression and localization of TRPV1 in the retina, at various eccentricities, of the vervet (Chlorocebus sabeus) monkey, using Western blots and immunohistochemistry. Our results demonstrate that TRPV1 is found mainly in the outer and inner plexiform layers, and in the retinal ganglion cell (RGC) layer with a higher density in the periphery. Co-immunolabeling of TRPV1 with parvalbumin, a primate horizontal cell marker, revealed a clear overlap of expression throughout the entire cell structure with most prominent staining in the cell body membrane and synaptic terminals. Furthermore, double labeling of TRPV1 and syntaxin was found throughout amacrine cells in the inner plexiform layer. Finally, double staining of TRPV1 and Brn3a allowed us to confirm its previously reported expression in the cell bodies and dendrites of RGCs. The presence of TRPV1 in the horizontal pathway suggests a function of this receptor in lateral inhibition between photoreceptors through the horizontal cells, and between bipolar cells through amacrine cells. |
| Databáze: | OpenAIRE |
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