K-ras mutations, rectal crypt cells proliferation, and meat consumption in patients with left-sided colorectal carcinoma
| ISSN: | 0959-8278 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4db4b42690079dc17697d80626a3c592 https://pubmed.ncbi.nlm.nih.gov/10777009 |
| Přírůstkové číslo: | edsair.doi.dedup.....4db4b42690079dc17697d80626a3c592 |
| Autor: | H J Kennedy, William Stebbings, Ian T. Johnson, J. M. Gee, M. Rhodes, J A Matthew, H. O'Brien, Chris T. M. Speakman, Mark Watson |
| Rok vydání: | 2000 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Meat Epidemiology Colorectal cancer DNA Mutational Analysis Rectum medicine.disease_cause Gastroenterology Polymerase Chain Reaction Risk Assessment Internal medicine Carcinoma medicine Humans Risk factor Aged Mutation Mucous Membrane business.industry Public Health Environmental and Occupational Health Middle Aged medicine.disease Diet medicine.anatomical_structure Cell Transformation Neoplastic Genes ras Oncology Red Meat Consumption Red meat Female Carcinogenesis business Colorectal Neoplasms Cell Division |
| Zdroj: | European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP). 9(1) |
| ISSN: | 0959-8278 |
| Popis: | It has been suggested that N-nitroso compounds derived from meat may increase the risk of K-ras mutations in the human colon. We sought evidence of associations between red meat consumption, frequency and type of K-ras mutations in resected tumours, and the rate of crypt cell proliferation (CCP) in the normal mucosa of patients with left-sided colorectal carcinoma. Meat consumption was assessed by food frequency questionnaire, and CCP was determined in rectal biopsies obtained prior to surgery. K-ras mutations in the resected tumours were determined using a PCR-based oligonucleotide hybridization assay. Fifteen K-ras mutations were detected in tumours from 43 patients; 13/15 in codon 12, 3/15 in codon 13, and 1/15 in both codons 12 and 13. All mutations were G-->A or G-->T transitions. There was no statistically significant difference between intakes of red meat in patients with a K-ras mutation (92.4 +/- 9.7 g/day) and those without (82.3 +/- 7.7 g/day). Rectal CCP was significantly higher in patients than in healthy controls, but there was no correlation with meat consumption or K-ras mutation. These data do not support the hypothesis that meat consumption is a risk factor for acquisition of K-ras mutations during colorectal carcinogenesis. |
| Databáze: | OpenAIRE |
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