Increased detection of Dirofilaria immitis antigen in cats after heat pretreatment of samples
| Autor: | Mason V. Reichard, Susan E. Little, Chris Adolph, Jennifer E. Thomas, Jeff M. Gruntmeir, Byron L. Blagburn |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hot Temperature 040301 veterinary sciences Dirofilaria immitis Population Animals Wild Cat Diseases Heat pretreatment Microbiology Specimen Handling 0403 veterinary science 03 medical and health sciences Blood serum Antigen Blood plasma Animals Small Animals education education.field_of_study CATS biology Dirofilaria immitis antigen 04 agricultural and veterinary sciences 030108 mycology & parasitology Antigens Helminth Immunology biology.protein Cats Dirofilariasis Antibody |
| Zdroj: | Journal of feline medicine and surgery. 19(10) |
| ISSN: | 1532-2750 |
| Popis: | Objectives To determine whether pretreating diagnostic samples with heat increases the detection of Dirofilaria immitis antigen in adult cats, we evaluated feline serum and plasma samples collected in heartworm-endemic areas of the southern United States. Methods Commercial microtiter well assays for detection of D immitis antigen were used to evaluate serum or plasma samples from 385 shelter and free-roaming cats from the southcentral and southeastern United States before and after heat treatment; commercial antibody tests were performed on a subset of samples. Results Prior to sample heat treatment, 1/220 (0.5%) shelter cats and 4/165 (2.4%) free-roaming cats had detectable D immitis antigen. After heat pretreatment, the detection rate increased to 13/220 (5.9%) and 13/165 (7.9%), respectively. Antibody reactive to D immitis was significantly more common ( P Conclusions and relevance Heat pretreatment of feline samples increased antigen detection by commercial assays for D immitis and improved overall concordance of antigen and antibody test results in antigen-positive samples in this population. Although further work to investigate the specificity of D immitis antigen assays when using pre-treated samples is warranted, this approach may be useful in the diagnosis of heartworm infection in individual cats and may increase the accuracy of surveys based on antigen detection. |
| Databáze: | OpenAIRE |
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