Detached and AttachedArabidopsisLeaf Assays Reveal Distinctive Defense Responses Against HemibiotrophicColletotrichumspp
| Autor: | Regan Kennedy, David L. Greenshields, Yangdou Wei, Lily Forseille, Guosheng Liu, Gopalan Selvaraj, Gary Peng |
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| Rok vydání: | 2007 |
| Předmět: |
Physiology
Mutant Arabidopsis Virulence Microbiology chemistry.chemical_compound Botany Colletotrichum Pathogen Gene Phylogeny Plant Diseases biology Arabidopsis Proteins fungi food and beverages General Medicine Fungi imperfecti Ethylenes biology.organism_classification Immunity Innate Plant Leaves chemistry Salicylic Acid Agronomy and Crop Science Salicylic acid Signal Transduction |
| Zdroj: | Molecular Plant-Microbe Interactions®. 20:1308-1319 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-20-10-1308 |
| Popis: | The agriculturally important genus Colletotrichum is an emerging model pathogen for studying defense in Arabidopsis. During the process of screening for novel pathogenic Colletotrichum isolates on Arabidopsis, we found significant differences in defense responses between detached and attached leaf assays. A near-adapted isolate Colletotrichum linicola A1 could launch a typical infection only on detached, but not attached, Arabidopsis leaves. Remarkably, resistance gene-like locus RCH1-mediated resistance in intact plants also was compromised in detached leaves during the attacks with the virulent reference isolate C. higginsianum. The differences in symptom development between the detached leaf and intact plant assays were further confirmed on defense-defective mutants following inoculation with C. higginsianum, where the greatest inconsistency occurred on ethylene-insensitive mutants. In intact Arabidopsis plants, both the salicylic acid- and ethylene-dependent pathways were required for resistance to C. higginsianum and were associated with induced expression of pathogenesis-related genes PR1 and PDF1.2. In contrast, disease symptom development in detached leaves appeared to be uncoupled from these defense pathways and more closely associated with senescence: an observation substantiated by coordinated gene expression analysis and disease symptom development, and chemically and genetically mimicking senescence. |
| Databáze: | OpenAIRE |
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