Simulation-based optimisation of thermodynamic conditions in the ESEM for dynamical in-situ study of spherical polyelectrolyte complex particles in their native state
| Autor: | Eva Tihlaříková, Jiří Maxa, Marek Bučko, Peter Gemeiner, Kamila Imrichová, Vilém Neděla |
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| Rok vydání: | 2019 |
| Předmět: |
010302 applied physics
Materials science Thermodynamic equilibrium Monte Carlo method 02 engineering and technology Electron 021001 nanoscience & nanotechnology 01 natural sciences Atomic and Molecular Physics and Optics Polyelectrolyte Electronic Optical and Magnetic Materials Characterization (materials science) law.invention law Chemical physics 0103 physical sciences Heat transfer Electron microscope 0210 nano-technology Instrumentation Environmental scanning electron microscope |
| Zdroj: | Ultramicroscopy. 211 |
| ISSN: | 1879-2723 |
| Popis: | We present a complex analysis and optimisation of dynamic conditions in the environmental scanning electron microscope (ESEM) to allow in-situ observation of extremely delicate wet bio-polymeric spherical particles in their native state. According to the results of gas flow and heat transfer simulations, we were able to develop an improved procedure leading to thermodynamic equilibrium between the sample and chamber environment. To quantify and hence minimise the extent of any sample deformation during specimen chamber pumping, a strength-stress analysis is used. Monte Carlo simulations of beam-gas, -water, and -sample interactions describe beam scattering, absorbed energy, interaction volume and the emission of signal electrons in the ESEM. Finally, we discuss sample damage as a result of drying and the production of beam-induced free radicals. Based on all experimental and simulation results we introduce a Delicate Sample Observation Strategy for the ESEM. We show how this strategy can be applied to the characterization of polyelectrolyte complex spherical particles containing immobilized recombinant cells E. coli overexpressing cyclohexanone monooxygenase, used as a model biocatalyst. We present the first native-state electron microscopy images of the viscous core of a halved polyelectrolyte complex capsule containing living cells. |
| Databáze: | OpenAIRE |
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