Ethanol has differential effects on rat neuron and thymocyte reactive oxygen species levels and cell viability
| Autor: | Craig M. Peters, Matthew J. Huentelman, Stephanie M Polutnik, William E Ervine, Peter Johnson |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Male
Free Radicals Cell Survival Immunology Thymus Gland Sulfides Antioxidants Rats Sprague-Dawley chemistry.chemical_compound Cerebellum medicine Animals Viability assay Cells Cultured Amitrole Alcohol dehydrogenase Fomepizole Neurons Pharmacology chemistry.chemical_classification Reactive oxygen species Ethanol biology Alcohol Dehydrogenase CYP2E1 Catalase Molecular biology Rats Allyl Compounds Cytochrome P-450 CYP2E1 Inhibitors Thymocyte medicine.anatomical_structure Biochemistry chemistry biology.protein Pyrazoles Female Neuron Reactive Oxygen Species |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology. 124:83-89 |
| ISSN: | 0742-8413 |
| DOI: | 10.1016/s0742-8413(99)00052-3 |
| Popis: | In rat thymocytes and cerebellar granule cells, reactive oxygen species (ROS) levels were increased and cell viability was decreased as a result of exposure to ethanol (up to 0.4%). Thymocytes showed larger increases in ROS levels, but neurons showed more pronounced decreases in cell viability. These parameters in neurons were relatively unaffected when the cells were incubated with ethanol in the presence of inhibitors of alcohol-oxidizing enzymes, but in thymocytes, the presence of diallyl sulfide (an inhibitor of alcohol-inducible cytochrome P450, CYP2E1) or 4-methylpyrazole (an inhibitor of CYP2E1 and alcohol dehydrogenase) caused decreases in ROS production from ethanol. In both cell types, the presence of 3-aminotriazole (an inhibitor of catalase) did not decrease ROS production from ethanol. These studies show that the cytotoxic effects of ethanol in neurons may not be the result of oxidative metabolism of ethanol, whereas in thymocytes, the cytotoxic effect of ethanol is principally a result of its oxidative metabolism. |
| Databáze: | OpenAIRE |
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