Rational Design of Enzyme‐Modified Electrodes for Optimized Bioelectrocatalytic Activity
| Autor: | Cristina Carucci, Bertrand Goudeau, Alexander Kuhn, Nicolas Mano, Lin Zhang, Sébastien Gounel, Stéphane Reculusa, Pauline Lefrançois |
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| Přispěvatelé: | Institut des Sciences Moléculaires (ISM), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Paul Pascal (CRPP), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), ANR: ANR-16-CE19-0001- 03,project BIO3, ANR-10-IDEX-03-02/10-LABX-0042,AMADEus,Advanced Materials by Design(2010), ANR: ANR-10-LABX-0042-AMADEUS,ANR-10-LABX-0042-AMADEUS, ANR-10-IDEX-0003-02/10-IDEX-0003,IDEX BORDEAUX,IdEx Bordeaux(2010), Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE19-0001,BIO3,Electrodes poreuses biocompatibles et biofonctionnelles pour des biopiles enzymatiques miniaturisées(2016), ANR-10-IDEX-0003,IDEX BORDEAUX,Initiative d'excellence de l'Université de Bordeaux(2010), ANR-10-LABX-0042,AMADEus,Advanced Materials by Design(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
bioelectrocatalysis
macroporous electrodes 02 engineering and technology 010402 general chemistry 01 natural sciences Catalysis Metal Active center Residue (chemistry) Monolayer Electrochemistry direct electron transfer Bilirubin oxidase cysteine bilirubin oxidase Chemistry Rational design [CHIM.CATA]Chemical Sciences/Catalysis 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences enzyme engineering visual_art Electrode visual_art.visual_art_medium 0210 nano-technology Cysteine |
| Zdroj: | ChemElectroChem ChemElectroChem, Weinheim : Wiley-VCH, 2019, 6 (19), pp.4980-4984. ⟨10.1002/celc.201901022⟩ |
| ISSN: | 2196-0216 |
| DOI: | 10.1002/celc.201901022⟩ |
| Popis: | International audience; The immobilization of bilirubin oxidase (BOD) on macroporous gold electrodes for the optimization of bioelectrocatalytic activity is described. A bilirubin oxidase mutant S362C (cys-BOD) engineered with a cysteine residue located on purpose at the enzyme surface close to the T1 active center was used. It allows the attachment in one-step of a self-assembled monolayer of the enzyme to gold through a reaction between the thiol group of the cysteine residue and the metal surface. BOD immobilization of wild type and S362C mutant in macroporous gold electrodes allowed high retention of activity and perfect control of the overall BOD loading due to the fine-tuning of the macroporous structure. The macroporous arrangement together with the use of cys-BOD makes these rationally designed enzyme-modified electrodes very promising candidates for high-performance bioelectrocatalytic devices with improved activity and stability. |
| Databáze: | OpenAIRE |
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