Natural variation in the expression and catalytic activity of a naringenin 7‐ O ‐methyltransferase influences antifungal defenses in diverse rice cultivars
Autor: | Yukinori Yabuta, Cynthia K. Holland, Atsushi Ishihara, Kotomi Ueno, Yutaka Okumoto, Georg Jander, Ryo Takata, Naoki Mori, Takashige Kitano, Makoto Ueno, Koichi Murata, Riko Yoshimoto, Naoki Ube, Masayoshi Teraishi |
---|---|
Rok vydání: | 2019 |
Předmět: |
0106 biological sciences
0301 basic medicine Naringenin Antifungal Agents Xanthomonas Burkholderia Cyclopentanes Plant Science 01 natural sciences Sakuranetin Comamonadaceae 03 medical and health sciences chemistry.chemical_compound Xanthomonas oryzae Ascomycota Fusarium Botany Genetics Burkholderia glumae Oxylipins Plant Diseases Flavonoids chemistry.chemical_classification Oryza sativa biology Phytoalexin Genetic Variation food and beverages Oryza Methyltransferases Cell Biology biology.organism_classification Oryza rufipogon 030104 developmental biology chemistry Flavanones Gibberella fujikuroi 010606 plant biology & botany |
Zdroj: | The Plant Journal. 101:1103-1117 |
ISSN: | 1365-313X 0960-7412 |
Popis: | Phytoalexins play a pivotal role in plant-pathogen interactions. Whereas leaves of rice (Oryza sativa) cultivar Nipponbare predominantly accumulated the phytoalexin sakuranetin after jasmonic acid induction, only very low amounts accumulated in the Kasalath cultivar. Sakuranetin is synthesized from naringenin by naringenin 7-O-methyltransferase (NOMT). Analysis of chromosome segment substitution lines and backcrossed inbred lines suggested that NOMT is the underlying cause of differential phytoalexin accumulation between Nipponbare and Kasalath. Indeed, both NOMT expression and NOMT enzymatic activity are lower in Kasalath than in Nipponbare. We identified a proline to threonine substitution in Kasalath relative to Nipponbare NOMT as the main cause of the lower enzymatic activity. Expanding this analysis to rice cultivars with varying amounts of sakuranetin collected from around the world showed that NOMT induction is correlated with sakuranetin accumulation. In bioassays with Pyricularia oryzae, Gibberella fujikuroi, Bipolaris oryzae, Burkholderia glumae, Xanthomonas oryzae, Erwinia chrysanthemi, Pseudomonas syringae, and Acidovorax avenae, naringenin was more effective against bacterial pathogens and sakuranetin was more effective against fungal pathogens. Therefore, the relative amounts of naringenin and sakuranetin may provide protection against specific pathogen profiles in different rice-growing environments. In a dendrogram of NOMT genes, those from low-sakuranetin-accumulating cultivars formed at least two clusters, only one of which involves the proline to threonine mutation, suggesting that the low sakuranetin chemotype was acquired more than once in cultivated rice. Strains of the wild rice species Oryza rufipogon also exhibited differential sakuranetin accumulation, indicating that this metabolic diversity predates rice domestication. |
Databáze: | OpenAIRE |
Externí odkaz: |