Prenatal diagnosis of congenital toxoplasmosis by duplex real-time PCR using fluorescence resonance energy transfer hybridization probes
| Autor: | Jean-Marc Costa, Stéphane Bretagne, Evelyne Gautier, Pauline Ernault |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty Amniotic fluid Antibodies Protozoan Prenatal diagnosis Biology Polymerase Chain Reaction Parasite load Toxoplasmosis Congenital Ultrasonography Prenatal law.invention Mice Pregnancy law Prenatal Diagnosis medicine Animals Humans Genetics (clinical) Polymerase chain reaction Fetus medicine.diagnostic_test Nucleic Acid Hybridization Obstetrics and Gynecology DNA Protozoan medicine.disease Toxoplasmosis Spectrometry Fluorescence Real-time polymerase chain reaction Amniocentesis Female Toxoplasma |
| Zdroj: | Prenatal Diagnosis. 21:85-88 |
| ISSN: | 1097-0223 0197-3851 |
| DOI: | 10.1002/1097-0223(200102)21:2<85::aid-pd18>3.0.co;2-1 |
| Popis: | The diagnosis of congenital toxoplasmosis frequently relies on PCR tests of amniotic fluid (AF). A duplex real-time quantitative PCR test based on fluorescence resonance energy transfer was developed to quantify the parasite load and to decrease the risk of contamination. An internal control based on the detection of 10 pg mouse DNA added to the AF was included to check for PCR efficiency. The relationship between the parasite load and the occurrence of ultrasonographic abnormalities in 87 samples of AF was analyzed. Seven AF (8%) had a parasitic load10(3); 14 (16%) had10(2)-or =10(3); 26 (30%) had10-or = 10(2); and 40 (46%) hador = 10 parasites/ml. Four of the six AF with cerebral ventriculomegaly had10(3) parasites/ml. The other two had 130 and 24 parasites/ml, respectively. No parasitic loads of10(3) parasites/ml and no ultrasonographic abnormalities were observed in the 11 AF with maternal toxoplasmosis in the third trimester. Therefore, there is a trend to associate high parasite count with ultrasonographic abnormality, but the main concern remains early maternal infection. The importance of quantification should be better evaluated with postnatal studies. The duplex LightCycler PCR test currently provides rapid and safe results. |
| Databáze: | OpenAIRE |
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