FoxM1 is associated with metastasis in colorectal cancer through induction of the epithelial-mesenchymal transition
| Autor: | Rui Chai, Jie Ma, Bao‑Ying Fei, Xujun He, Mei Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research epithelial-mesenchymal transition colorectal cancer Biology Metastasis 03 medical and health sciences 0302 clinical medicine medicine epithelial cadherin Epithelial–mesenchymal transition Gene knockdown Oncogene Cadherin Articles medicine.disease Molecular medicine digestive system diseases 030104 developmental biology Real-time polymerase chain reaction Oncology neuronal cadherin 030220 oncology & carcinogenesis forkhead box M1 Cancer research Immunohistochemistry |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | The aim of the present study was to investigate the role of forkhead box M1 (FoxM1) in epithelial-mesenchymal transition (EMT) and metastasis in colorectal cancer (CRC). Immunohistochemical assays were performed to detect FoxM1 and epithelial (E-) cadherin protein expression in 92 CRC, 61 colonic adenoma and 32 wild-type colonic tissue samples. Quantitative polymerase chain reaction (qPCR) assays were performed to determine the expression levels of FoxM1 and E-cadherin mRNAs in 30 CRC and adjacent normal mucosal tissues. RNA interference was used to knock down endogenous FoxM1 expression in CRC cell lines, and the migratory and invasive capacity of the CRC cells was analyzed. The expression of FoxM1, E-cadherin and neuronal (N-) cadherin in the CRC cell lines was evaluated using qPCR and Western blot analysis. The relative expression levels of FoxM1 mRNA and protein were significantly increased in the CRC tissues compared with those in the colonic adenoma and wild-type mucosal tissue samples (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|