Eosinophil cationic protein mRNA expression in children with bronchial asthma
| Autor: | Shi Xz, Xiaoying Li, Cai Zf, Song Hx, Leping Li, Yu Hy, Xiangyan Liu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
Mrna expression education law.invention Pathogenesis fluids and secretions law Healthy control Genetics Medicine Humans RNA Messenger Child Molecular Biology Polymerase chain reaction Bronchial hyperreactivity Asthma Eosinophil cationic protein business.industry Eosinophil Cationic Protein General Medicine respiratory system medicine.disease respiratory tract diseases Eosinophils Immunology Female Bronchial Hyperreactivity business |
| Zdroj: | Genetics and molecular research : GMR. 14(4) |
| ISSN: | 1676-5680 |
| Popis: | Studies have shown that eosinophils are closely related to pathogenesis of bronchial asthma. Eosinophils release eosinophil cationic protein (ECP), which plays an important role in infection and allergic reactions. Serum ECP mRNA expression in children with bronchial asthma has not been adequately investigated. We analyzed serum ECP mRNA expression in 63 children with bronchial asthma and 21 healthy children by using reverse-transcriptase polymerase chain reaction to understand the role of ECP in children with bronchial asthma. The children with bronchial asthma were segregated into acute-phase and stable-phase groups, based on the severity of the illness. Serum ECP mRNA expression in children with bronchial asthma (0.375 ± 0.04) was significantly higher than that in healthy controls (0.20 ± 0.02; P < 0.05). Additionally, children in the acute-phase group showed higher ECP mRNA expression level (0.44 ± 0.06) than those in the stable-phase (0.31 ± 0.03) and healthy control groups (0.20 ± 0.02; P < 0.05), while the level in the stable-phase (0.31 ± 0.03) was markedly higher than that in the healthy control group (0.20 ± 0.02; P < 0.05). Detection of serum ECP mRNA expression level has possible applications in the diagnosis and treatment of children with bronchial asthma. |
| Databáze: | OpenAIRE |
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