Increased Ocular Levels of MicroRNA-148a in Cases of Retinal Detachment Promote Epithelial-Mesenchymal Transition
| Autor: | Hiroko Terasaki, Fuxiang Ye, Takeshi Iwase, Yasuki Ito, Shunsuke Yasuda, Tetsu Asami, Norie Nonobe, Toshiyuki Matsuura, Shinji Ueno, Shiang-Jyi Hwang, Taichi Tsunekawa, Hiroki Kaneko, Kei Takayama, Akiko Higuchi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Epithelial-Mesenchymal Transition genetic structures Blotting Western Real-Time Polymerase Chain Reaction 03 medical and health sciences Young Adult Blood serum Cell Movement Vitrectomy microRNA medicine Humans Epithelial–mesenchymal transition Aged business.industry Retinal Detachment Retinal detachment Transfection Middle Aged medicine.disease Molecular biology eye diseases Epithelium Blot Vitreous Body MicroRNAs 030104 developmental biology medicine.anatomical_structure Real-time polymerase chain reaction embryonic structures RNA Female sense organs business |
| Zdroj: | Investigative ophthalmologyvisual science. 57(6) |
| ISSN: | 1552-5783 |
| Popis: | Purpose The purpose of this study was to determine microRNA expression in vitreous and subretinal fluid (SRF) samples from patients with retinal detachment (RD). The pathological importance of the identified microRNA transcript levels was analyzed in vitro. Methods Vitreous fluid was collected from 10 patients with macular hole (MH), vitreomacular traction syndrome (VMTS), or foveoschisis and from 11 patients with RD. Subretinal fluid was collected from 7 patients with RD. Of these, blood serum was collected in 4 patients. MicroRNA microarray profiling was performed to identify microRNA transcripts that were present in vitreous fluid, and more redundantly detected in SRF, of patients with RD, but not detected in control eyes. Western blotting and scratch assays were performed in ARPE-19 cells and primary human RPE cell lines transfected with microRNA to elucidate the effect of identified microRNA transcripts on epithelial-mesenchymal transition (EMT). Results MicroRNA microarray profiling revealed that hsa-miR-148a-3p was the most redundantly detected transcript in SRF and vitreous fluid from patients with RD, but not those with the other diseases. Expression levels of hsa-miR-148a-3p were higher in SRF samples than in blood serum samples in 3 out of 4 patients. Following hsa-miR-148a-3p mimic transfection, ARPE-19 and human RPE cells demonstrated increased expression of α-smooth muscle actin by Western blotting and increased migration ability during scratch assays. Conclusions The results of the present study indicate that hsa-miR-148a-3p was specifically detected in RD and promotes EMT in RPE. |
| Databáze: | OpenAIRE |
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