Tissue tropism of African horsesickness virus in the chicken embryo demonstrated with the avidin-biotin complex immunoperoxidase method
| Autor: | B. J. Erasmus, L. H. Maartens, Sarah J. Clift |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Time Factors Connective tissue Biotin Spleen Chick Embryo Biology Antibodies Viral Immunoenzyme Techniques Immunolabeling African Horse Sickness medicine African Horse Sickness Virus Animals Horses Antigens Viral Tropism General Veterinary Immunoperoxidase Endothelial Cells Mononuclear phagocyte system Avidin Virology Specific Pathogen-Free Organisms Viral Tropism medicine.anatomical_structure Tissue tropism Immunohistochemistry Rabbits Chickens |
| Zdroj: | Veterinary pathology. 48(6) |
| ISSN: | 1544-2217 |
| Popis: | In horses, African horsesickness virus (AHSV) exhibits marked tropism for certain microvascular endothelia and components of the mononuclear phagocyte system. In this study, the tropism of a field isolate of AHSV serotype 5 was studied in 24 chicken embryos. Histopathology on embryonic tissues harvested with 12 hour intervals revealed progressive changes associated with endothelial damage. Immunolabeling demonstrated viral antigens in the microvascular endothelium of the spleen, lungs, and the mesenchymal connective tissue at the base of the neck, from 24 hours post inoculation. Subsequently, specific immunolabeling increased steadily in endothelia of these and other tissues such as skeletal and cardiac muscle, gastrointestinal smooth muscle, mesonephric glomeruli, liver, subcutis and feathers. Positive immunolabeling was also occasionally observed in circulating mononuclear cells and in Kupffer cells in the liver. It was concluded, that this isolate of AHSV displayed similar tissue tropism in the chicken embryo as in the horse. |
| Databáze: | OpenAIRE |
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