Characterization of an l-α,β-diaminopropionic acid polymer with comb-like structure isolated from a poly(ε-l-lysine)-producing Streptomyces sp
| Autor: | Masayuki Saimura, Munenori Takehara, Kazuya Yamanaka, Tatsuki Matsunaga, Yoshinao Kato, Haruka Inaba, Shogo Muro |
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| Rok vydání: | 2021 |
| Předmět: |
Polymers
Stereochemistry Lysine Applied Microbiology and Biotechnology Streptomyces 03 medical and health sciences chemistry.chemical_compound Glycerol Polylysine 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Strain (chemistry) 030306 microbiology Cationic polymerization General Medicine Biodegradation biology.organism_classification Amino acid chemistry Fermentation beta-Alanine Bacteria Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 105:3145-3157 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-021-11257-3 |
| Popis: | Polymers of basic amino acids function as polycationic compounds under physiological conditions and exhibit intriguing biological properties, such as antimicrobial and antiviral activities, immunopotentiating ability, and DNA-binding activity. Poly(e-L-lysine) (e-PL) produced by some strains of Streptomyces spp. is a cationic homopolymer of L-lysine linking between e-amino and α-carboxylic acid functional groups and has been used as a food preservative based on its biocompatibility and biodegradability. An e-PL-producing strain of Streptomyces sp. USE-33 was found to secrete a novel polycationic substance into its culture broth along with e-PL. High-performance liquid chromatography analyses and one- and two-dimensional 1H and 13C nuclear magnetic resonance (NMR) experiments, accompanied by NMR titration studies, revealed that the secreted substance was poly[β-(L-diaminopropionyl-L-diaminopropionic acid)], PAP, characterized by an isopeptide backbone linking between the β-amino and α-carboxylic acid groups of L-α,β-diaminopropionic acid (L-Dpr) with pendent L-Dpr residues. PAP had a molecular weight of 500 to 1400, and copolymers composed of the two amino acids L-Dpr and L-lysine were not detected in the producer strain USE-33. The strain coproduced high levels of the two poly(amino acid)s in the presence of glycerol, citrate, and ammonium sulfate at pH 4.0 in a two-stage cultivation procedure. PAP exhibited strong inhibitory activities against several yeasts and weaker activities against bacteria than e-PL. PAP may share a number of biological functions with e-PL, and the use of PAP along with e-PL has potential as a specific and advanced material for technical applications in various fields.Key points• Novel cationic poly(amino acid) was found in an e-PL-producing Streptomyces species.• The l-α,β-diaminopropionic acid polymer was characterized by a comb-like structure.• The novel poly(amino acid), PAP, exhibited antibacterial and antifungal activities. |
| Databáze: | OpenAIRE |
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