Cutaneous pythiosis in a donkey (Equus asinus) in Brazil
| Autor: | Roberio Gomes Olinda, P. R. Firmino, Franklin Riet-Correa, Luciano Nakazato, Antônio Flávio Medeiros Dantas, Lisanka A. Maia, Tenório F. Araújo, Eldinê Gomes de Miranda Neto |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty 040301 veterinary sciences 030106 microbiology Pythium Folliculitis Pythium insidiosum Grocott's methenamine silver stain 0403 veterinary science 03 medical and health sciences Pythiosis biology.animal medicine Animals Skin Diseases Parasitic Inflammation General Veterinary biology Equidae Sequence Analysis DNA 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Equus asinus Donkey Brazil Immunostaining |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 28:436-439 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | Our study describes the clinical, epidemiologic, pathologic, immunohistochemical, and molecular aspects PCR of a case of cutaneous pythiosis in a donkey ( Equus asinus) from Brazil. During a dry period, the animal grazed for 4 months around a pond where the vegetation remained green. Skin lesions were nodular, multifocal, and disseminated, mainly involving the legs, ventral chest, and mammary gland. On cut surface, there were multifocal to coalescent discrete yellow foci, and occasional small cavitations with a few kunkers. Ulcerative nodular pyogranulomatous and eosinophilic dermatitis with folliculitis and furunculosis were observed histologically. Hyphae were observed in sections stained with Gomori methenamine silver. Immunohistochemistry with Pythium insidiosum antibodies yielded strong immunostaining of hyphae. P. insidiosum DNA was extracted from tissues in paraffin blocks by amplification of a fragment of 105 bp, which targets the 5.8S ribosomal gene. After the diagnosis of pythiosis, the larger skin lesions were excised and treated as second intention healing wounds, which were completely healed 30 days after resection. Small skin lesions regressed spontaneously in ~60 days. The granulomatous inflammation and outcome of the disease in this donkey were similar to cases of pythiosis in cattle. |
| Databáze: | OpenAIRE |
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