Identification and quantitative detection of Legionella spp. in various aquatic environments by real-time PCR assay
| Autor: | Shu-Min Shen, Po-Min Kao, Yi-Chou Chiu, Min-Che Tung, Cheng-Yu She, Yu-Li Huang, Wen-Chien Huang, Bing-Mu Hsu |
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| Rok vydání: | 2013 |
| Předmět: |
Veterinary medicine
Legionella Health Toxicology and Mutagenesis Taiwan Biology Real-Time Polymerase Chain Reaction River water Legionella pneumophila Microbiology Rivers Environmental water Environmental Chemistry Ecotoxicology Phylogeny Fluorescent Dyes Aquatic ecosystem General Medicine bacterial infections and mycoses biology.organism_classification Pollution respiratory tract diseases Real-time polymerase chain reaction bacteria Water treatment Water Microbiology |
| Zdroj: | Environmental Science and Pollution Research. 20:6128-6137 |
| ISSN: | 1614-7499 0944-1344 |
| DOI: | 10.1007/s11356-013-1534-z |
| Popis: | In this study, a SYBR green quantitative real-time PCR was developed to quantify and detect the Legionella spp. in various environmental water samples. The water samples were taken from watershed, water treatment plant, and thermal spring area in Taiwan. Legionella was detected in 13.6 % (24/176), and the detection rate for river water, raw drinking water, and thermal spring water was 10, 21.4, and 16.6 %, respectively. Using real-time PCR, concentration of Legionella spp. in detected samples ranged between 9.75 × 10(4) and 3.47 × 10(5) cells/L in river water, 6.92 × 10(4) and 4.29 × 10(5) cells/L in raw drinking water, and 5.71 × 10(4) and 2.12 × 10(6) cells/L for thermal spring water samples. The identified species included Legionella pneumophila (20.8 %), Legionella jordanis (4.2 %), Legionella nautarum (4.2 %), Legionella sp. (4.2 %), and uncultured Legionella sp. (66.6 %). The presence of L. pneumophila in aquatic environments suggested a potential public health threat that must be further examined. |
| Databáze: | OpenAIRE |
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