Staphylococcal Enterotoxin B: Solid-Phase Radioimmunoassay
| Autor: | Howard M. Johnson, Joann A. Bukovic, Peter E. Kauffman, J. T. Peeler |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1971 |
| Předmět: |
Immunodiffusion
Food and Deterioration General Immunology and Microbiology Immune Sera Staphylococcus Radioimmunoassay General Medicine Cross Reactions General Biochemistry Genetics and Molecular Biology Antigen-Antibody Reactions Enterotoxins Iodine Isotopes Methods Animals Polystyrenes Rabbits General Pharmacology Toxicology and Pharmaceutics Food Analysis Mathematics Protein Binding |
| Popis: | An immunoassay employing 125 I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated polystyrene tubes. Unlabeled enterotoxin competed with 125 I-labeled enterotoxin for antibody-combining sites. The uptake of 125 I-labeled toxin reflected the concentration of unlabeled toxin present. The test is sensitive to 1 to 5 ng of purified and crude enterotoxin B per ml, and cross-reactions with heterologous enterotoxins did not interfere with the specificity. This test possesses the combination of sensitivity and objectivity absent in current methods for assaying enterotoxin and provides a model for investigating other enterotoxin serotypes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|