Potentiation of IL-2-induced t-cell proliferation by retinoids
Autor: | Arabella B. Tilden, Eddie W. Lamon, Michael P. Everson, Xiao L. Jiang, Dirck L. Dillehay |
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Rok vydání: | 1992 |
Předmět: |
Interleukin 2
medicine.medical_specialty medicine.drug_class medicine.medical_treatment T cell T-Lymphocytes Immunology Stimulation Tretinoin Biology Lymphocyte Activation Mice Internal medicine medicine Splenocyte Animals Retinoid Receptor Cells Cultured Pharmacology Mice Inbred BALB C Drug Synergism Receptors Interleukin-2 Recombinant Proteins Cytokine medicine.anatomical_structure Endocrinology Concanavalin A biology.protein Interleukin-2 Female Interleukin-4 medicine.drug |
Zdroj: | International journal of immunopharmacology. 14(2) |
ISSN: | 0192-0561 |
Popis: | We evaluated the capacity of retinoids to potentiate proliferative responses of murine T-cells to recombinant human interleukin 2 (rIL-2). Concanavalin A (Con A) prestimulated spleen cells responded in a dose-dependent manner to added rIL-2. All-trans-retinoic acid (RA) at 10(-8) M potentiated the proliferative response by fivefold at saturating levels of IL-2. In similar experiments, two closely related retinamides, all-trans-(phenyl)retinamide (PR) and N-(4-hydroxyphenyl)retinamide (4-HPR), also potentiated murine splenocyte rIL-2 responses. Potentiation of IL-2-induced proliferation was dose-responsive to the concentration of added retinoid with peak potentiation occurring at 10(-10) - 10(-8) M in the presence of 10 U/ml rIL-2. Significant potentiation was observed at retinoid concentrations as low as 10(-14) M. Fluorescence flow cytometry of the responding cells revealed that among L3T4+, Lyt-2+ or total T-cells, at 72 h following Con A stimulation, essentially all of the cells expressed IL-2 receptors (IL-2R). This apparently represents near maximum IL-2R expression and treatment of the cells with retinoids did not increase IL-2R expression at that time point. The potentiation of IL-2 responses by retinoids was also observed with IL-2-dependent HT-2 cells, 98% of which were IL-2R positive. HT-2 proliferative responses to rIL-2 were potentiated as much as fourfold by 10(-10) M RA. HT-2 proliferative responses to rIL-2 were potentiated by all three retinoids dose dependently. Significant potentiation was observed with as little as 10(-14) M retinoid. Retinoids in the absence of IL-2 induced no proliferative responses. These data suggest that retinoids can augment the capacity of IL-2 to induce T-cell proliferation using Con A-activated murine splenic T-cell blasts and a long-term-cultured T-cell line. |
Databáze: | OpenAIRE |
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