Molecular characterization of Brazilian wild-type strains of bovine respiratory syncytial virus reveals genetic diversity and a putative new subgroup of the virus
| Autor: | Fernanda Louise Pereira, Flávia Possatti, Raquel Arruda Leme, Alais Maria Dall Agnol, Alice Fernandes Alfieri, Luciana C. Balbo, Amauri Alcindo Alfieri |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Veterinary medicine Bovine respiratory syncytial virus Bovine respiratory disease Cattle Diseases Respiratory Syncytial Virus Bovine bovine orthopneumovirus Respiratory Syncytial Virus Infections Polymerase Chain Reaction Virus Article brd Orthopneumovirus SF600-1100 medicine Animals Phylogeny Genetic diversity calf General Veterinary biology bovine Wild type Genetic Variation medicine.disease biology.organism_classification Virology bovine respiratory disease Cattle Female brsv Sequence Analysis Brazil |
| Zdroj: | Veterinary Quarterly, Vol 40, Iss 1, Pp 83-96 (2020) The Veterinary Quarterly |
| DOI: | 10.6084/m9.figshare.11940588.v1 |
| Popis: | Background: Bovine orthopneumovirus, formerly known as bovine respiratory syncytial virus (BRSV), is frequently associated with bovine respiratory disease (BRD). Aim: To perform the molecular characterization of the G and F proteins of Brazilian wild-type BRSV strains derived from bovine respiratory infections in both beef and dairy cattle. Materials and Methods: Ten BRSV strains derived from a dairy heifer rearing unit (n = 3) in 2011 and steers of three other feedlots (n = 7) in 2014 and 2015 were analyzed. For the BRSV G and F partial gene amplifications, RT-nested-PCR assays were performed with sequencing in both directions with forward and reverse primers used. Results: The G gene-based analysis revealed that two strains were highly similar to the BRSV sequences representative of subgroup III, including the Bayovac vaccine strain. However, the remaining seven Brazilian BRSV strains were diverse when compared with strains representative of the BRSV I to VIII subgroups. The central hydrophobic region of the Brazilian BRSV G gene showed the replacement of conserved cysteines and other residues of importance to antibody reactivity. The deduced F gene amino acid sequences from the Brazilian BRSV strains showed changes that were absent in the representative sequences of the known subgroups. Viral isolation on the nasopharyngeal swab suspensions failed to isolate BRSV. Conclusion: Results suggest that these strains represent a putative new subgroup of BRSV with mutations observed in the immunodominant region of the G protein. However, further studies on these Brazilian BRSV strains should be performed to establish their pathogenic potential. |
| Databáze: | OpenAIRE |
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