A SpoT polymorphism correlates with chill stress survival and is prevalent in clinical isolates of Campylobacter jejuni

Autor: M.A. Smits, M. C. van der Hulst-van Arkel, A.G. de Boer, P.P.L.A. de Leeuw, H. C. A. Widjaja, M.N. Nierop Groot, W. van Pelt, F.J. van der Wal
Jazyk: angličtina
Rok vydání: 2014
Předmět:
broiler meat
Animal Nutrition
netherlands
medicine.disease_cause
Feces
chicken carcasses
Sigma factor
Genotype
Campylobacter Infections
Phylogeny
Genetics
poultry
Bacteriologie
food and beverages
htra degp protein
Bacteriology
Host Pathogen Interaction & Diagnostics
General Medicine
Diervoeding
Cold Temperature
virulence-associated phenotypes
Wageningen Livestock Research
Genetic Markers
Meat
Molecular Sequence Data
Biology
Campylobacter jejuni
Polymorphism
Single Nucleotide
Microbiology
strains
Bacterial Proteins
Stress
Physiological
expression
medicine
SNP
Animals
Gene
Escherichia coli
Poultry Diseases
Host Pathogen Interaction & Diagnostics
Microbial Viability
Bacteriology
Sequence Analysis
DNA
biology.organism_classification
colonization
Host Pathogen Interactie & Diagnostiek
Bacteriologie
Host Pathogen Interactie & Diagnostiek
escherichia-coli
CVI - Divisie Bacteriologie en TSE's
Food Technology
rpoN
Animal Science and Zoology
Chickens
Zdroj: Poultry Science 93 (2014) 11
Poultry Science, 93(11), 2900-2909
ISSN: 0032-5791
Popis: Resistance of Campylobacter jejuni to environmental stress is regarded as a risk factor for the transmission of C. jejuni from poultry or poultry products to humans. So far, the mechanisms underlying the capacity of C. jejuni to survive environmental stress conditions are not fully understood. In this study, we searched for polymorphisms in C. jejuni genes, potentially involved in resistance to chill stress. To this end, we assessed 3 groups of C. jejuni isolates (clinical, retail chicken meat, and feces) for survival of experimentally induced chill stress. For each isolate we sequenced 3 genes encoding the C. jejuni sigma factors FliA, RpoD, and RpoN as well as the genes for the transcriptional regulator SpoT and the periplasmic protein HtrA. Data suggest a higher prevalence of a specific polymorphism in spoT in clinical isolates compared with poultry meat or farm isolates. Moreover, this genotype correlated with enhanced survival of chill stress. The observation that the prevalence of this SNP is relatively high in clinical isolates, which most likely have been exposed to multiple forms of stress, suggest that this SNP may be a biomarker for enhanced survival of stress.
Databáze: OpenAIRE
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