Anticancer and antioxidant activities of Nannochloropsis oculata and Chlorella sp. extracts in co-application with silver nanoparticle
| Autor: | Habsah Mohamad, Hanaa Ali Hussein, Maziah Mohd Ghazaly, A.A. Laith, Mohd Azmuddin Abdullah |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
medicine.medical_treatment Silver nanoparticle 02 engineering and technology 010501 environmental sciences 01 natural sciences chemistry.chemical_compound Co-application Antioxidant activity medicine Microalgae Cytotoxic T cell Cytotoxicity lcsh:Science (General) IC50 0105 earth and related environmental sciences Anticancer formulation Natural products Multidisciplinary Chloroform Chromatography 021001 nanoscience & nanotechnology chemistry Apoptosis Vero cell 0210 nano-technology lcsh:Q1-390 |
| Zdroj: | Journal of King Saud University: Science, Vol 32, Iss 8, Pp 3486-3494 (2020) |
| ISSN: | 1018-3647 |
| Popis: | This study examined the formulation of Microalgal Chloroform Extracts (MEs) and Silver nanoparticles (AgNPs) co-application for anticancer activity against MCF-7 and 4T1 cells, without affecting the non-cancerous Vero cell-lines. The concentration, ratios and duration of treatments were optimized, and the flow cytometric and cell cycle analyses were carried out. The metabolites based on Gas Chromatography-Mass Spectrometry (GC–MS) were determined and the antioxidant activities evaluated. The main compounds detected in Chlorella sp. and N. oculata, respectively, were hexanedioic acid, bis (2-ethylhexyl) ester (23.94, 36.47%), neophytadiene (16.82, 4.79%), eicosane (4.37, 15.04%), hexatriacontane (0, 12.77%), and 13-Docosenamide, (Z) (9.22, 0%). The AgNPs-N. oculata-CHL at (w/w) 1.5:1 and 2:1 ratios (w/w) exhibited cytotoxic IC50 of 10.47 and 17.78 µg/mL on MCF-7 cells; and 79.43 and 52.7 µg/mL against 4T1 cells after 72 h, respectively. The AgNPs-Chlorella sp.-CHL at 1:1 and 2:1 ratios exhibited IC50 of 19.05 and 14.45 µg/mL against MCF-7 cells; and 79.43 and 50.11 µg/mL on 4T1 cells after 72 h, respectively. There was no cytotoxicity against Vero cells at any of the treatments tested. The co-applications showed higher early and late apoptotic events and significant increase in sub G1 phase as compared to the single-applications. At the 2:1 ratio, the strongest anti-oxidant activities were shown by the AgNPs-Chlorella sp.-CHL (IC50 2.11 mg/mL) and N. oculata-CHL (IC50 2.98 mg/mL), as compared to the AgNPs-T. suecica-CHL (IC50 1.77 mg/mL). The AgNPs-MEs co-application exerted high anticancer and antioxidant activities, with no cytotoxic activity against Vero cells. The formulation could lead to the development of potent therapeutic agents against breast cancer with reduced or no side effect. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|