Evaluation the binding of chelerythrine, a potentially harmful toxin, with bovine serum albumin
| Autor: | Fuyun Ji, Yiwei Wu, Jingjing Xu, Chunlei Zhu, Wencheng Zhang, Yizhong Shen, Yaping Wang, Ruyu Xue, Yingwang Ye, Zeyu Wu, Zhi Zheng |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Protein Conformation
alpha-Helical Toxicology medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology In vivo medicine Distribution (pharmacology) Animals Bovine serum albumin 030304 developmental biology Benzophenanthridines 0303 health sciences Binding Sites integumentary system biology Toxin Chemistry Alkaloid Circular Dichroism Serum Albumin Bovine 04 agricultural and veterinary sciences General Medicine Metabolism 040401 food science Chelerythrine Spectrometry Fluorescence Biochemistry Toxicity biology.protein Thermodynamics Cattle Food Science Protein Binding |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 135 |
| ISSN: | 1873-6351 |
| Popis: | Chelerythrine (CHE), a benzophenanthridine alkaloid, is usually used as a nutritional and functional additive in variety of health foods. However, it should be paid enough attention because of its potential toxicity to human health. In this work, the binding mechanism of CHE with bovine serum albumin (BSA) was systematically investigated with spectroscopic approaches. The results showed that the mixture of BSA with CHE could spontaneously cause the formation of BSA-CHE complex through electrostatic interaction under simulative physiological conditions (0.01 mol L−1 Tris-HCl, 0.015 mol L−1 NaCl, pH = 7.4). Site marker competitive displacement experiments exhibited that CHE was primarily bound to the hydrophobic pocket of the site II (subdomain IIIA) of BSA. It has been reported that the binding of small functional molecules to serum albumins remarkably impacts their absorption, distribution, metabolism, conformation, and excretion features. Therefore, this study might be helpful for human to have an in-depth understanding of the biological effect of CHE in vivo and guide human to take it safely and reasonably. |
| Databáze: | OpenAIRE |
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