Proteomics analysis of human serum of patients with non-small-cell lung cancer reveals proteins as diagnostic biomarker candidates
| Autor: | Marina Di Domenico, Federica Pinto, Mario Santini, Arberesha Bexheti-Ferati, Lucio Quagliuolo, Annalisa Carlucci, Kenan Ferati, Mario Coppola, Mariarosaria Boccellino, Vincenzo Ieluzzi, Chiara Fariello, Antonio Giordano, Alfonso Giovane |
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| Přispěvatelé: | Boccellino, Mariarosaria, Pinto, Federica, Ieluzzi, Vincenzo, Giovane, Alfonso, Quagliuolo, Lucio, Fariello, Chiara, Coppola, Mario, Carlucci, Annalisa, Santini, Mario, Ferati, Kenan, Bexheti-Ferati, Arbëresha, Giordano, Antonio, Di Domenico, Marina |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Proteomics 0301 basic medicine Lung Neoplasms α2 macroglobulin Physiology Clinical Biochemistry Quantitative proteomics Adenocarcinoma of Lung NSCLC 03 medical and health sciences 0302 clinical medicine AMBP HPLC-MS/MS analysis SERPINA1 Carcinoma Non-Small-Cell Lung Alpha-Globulins Biomarkers Tumor medicine Humans Protease Inhibitors Endopeptidase inhibitor activity Lung cancer Survival rate Early Detection of Cancer Aged business.industry Cancer Cell Biology Middle Aged medicine.disease Neoplasm Proteins Macroglobulin 030104 developmental biology HPLC-MS/MS analysi alpha 1-Antitrypsin 030220 oncology & carcinogenesis Cancer research Adenocarcinoma Female business |
| Popis: | Non-small-cell lung carcinomas (NSCLC) is the most common type of lung cancer and it has a poor prognosis, because overall survival after 5 years is 20-25% for all stages. Thus, it is extremely important to increase the survival rate in the early stages NSCLC by focusing on novel screening tests of cancer identifying specific biomarkers expression associated with a more accurate tumor staging and patient prognosis. In this study, we focused our attention on quantitative proteomics of three heavily glycosylated serum proteins: AMBP,α2 macroglobulin, and SERPINA1. In particular, we analyzed serum samples from 20 NSCLC lung adenocarcinoma cancer patients in early and advanced stages, and 10 healthy donors to obtain a relative quantification through the MRM analysis of these proteins that have shown to be markers of cancer development and progression. AMBP, α2 macroglobulin, and SERPINA1 were chosen because all of them possess endopeptidase inhibitor activity and play key roles in cancer. We observe a variation in the expression of these proteins linked to the stage of the disease. Therefore, we believe that proteins like α2 macroglobulin, αmicroglobulin/bikunin, and SERPINA1 could be useful biomarkers for early detection of lung cancer and in monitoring its evolution. |
| Databáze: | OpenAIRE |
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