Temperature-induced reorganisation of Schistocephalus solidus (Cestoda) proteome during the transition to the warm-blooded host
| Autor: | Victor G. Zgoda, Polina Drozdova, Olga V. Balan, Albina Kochneva, Ekaterina Borvinskaya |
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| Rok vydání: | 2021 |
| Předmět: |
Hot Temperature
QH301-705.5 proteome Science Cestoda Zoology plerocercoid Gasterosteus schistocephalus solidus Solidus General Biochemistry Genetics and Molecular Biology Host-Parasite Interactions Fish Diseases Ribosomal protein Plerocercoid Animals Parasite hosting Biology (General) cestoda biology Host (biology) Chemistry Viral tegument biology.organism_classification Smegmamorpha Gluconeogenesis Warm-blooded Biochemistry parasite Schistocephalus solidus General Agricultural and Biological Sciences Research Article |
| Zdroj: | Biology Open, Vol 10, Iss 11 (2021) Biology Open article-version (VoR) Version of Record |
| Popis: | The protein composition of the cestode Schistocephalus solidus was measured in an experiment simulating the trophic transmission of the parasite from a cold-blooded to a warm-blooded host. The first hour of host colonisation was studied in a model experiment, in which sticklebacks Gasterosteus aculeatus infected with S. solidus were heated at 40°C for 1 h. As a result, a decrease in the content of one tegument protein was detected in the plerocercoids of S. solidus. Sexual maturation of the parasites was initiated in an experiment where S. solidus larvae were taken from fish and cultured in vitro at 40°C for 48 h. Temperature-independent changes in the parasite proteome were investigated by incubating plerocercoids at 22°C for 48 h in culture medium. Analysis of the proteome allowed us to distinguish the temperature-induced genes of S. solidus, as well as to specify the molecular markers of the plerocercoid and adult worms. The main conclusion of the study is that the key enzymes of long-term metabolic changes (glycogen consumption, protein production, etc.) in parasites during colonisation of a warm-blooded host are induced by temperature. Summary: The study focuses on the processes that determine the survival of parasites in warm-blooded animals and demonstrates the potential for the application of proteomics in veterinary medicine. |
| Databáze: | OpenAIRE |
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