Creutzfeldt-Jakob disease with amyloid angiopathy: diagnosis by immunological analyses and transmission experiments
| Autor: | Tetsuyuki Kitamoto, Katsumi Doh-ura, Jun Tateishi, J. W. Boellaard, J. Peiffer |
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| Rok vydání: | 1992 |
| Předmět: |
Electrophoresis
Male Gene isoform Pathology medicine.medical_specialty Prions animal diseases Blotting Western Mice Inbred Strains Disease Creutzfeldt-Jakob Syndrome Pathology and Forensic Medicine Incubation period Mice Cellular and Molecular Neuroscience mental disorders Animals Humans Medicine Pathological Aged Amyloid beta-Peptides Paraffin Embedding biology business.industry Immunohistochemistry nervous system diseases Blot Cerebral Amyloid Angiopathy Mice Inbred CBA biology.protein Female Neurology (clinical) Antibody business Immunostaining |
| Zdroj: | Acta Neuropathologica. 83:559-563 |
| ISSN: | 1432-0533 0001-6322 |
| DOI: | 10.1007/bf00310037 |
| Popis: | It was difficult to make a definite pathological diagnosis in a 73-year-old man with Creutzfeldt-Jakob disease (CJD) due to extensive amyloid angiopathy which lacked any severe spongiform changes. Immunostaining using anti-prion protein (PrP) antibody revealed fine granular deposits in the gray matter, after hydrolytic autoclaving pretreatment on tissue sections. Western blotting also revealed an abnormal isoform of PrP, but PrP gene analysis did not show any abnormalities. The primary transmission experiments were repeated three times and induced spongiform encephalopathy in a few mice after a long incubation period. |
| Databáze: | OpenAIRE |
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