High-throughput proteomics processing of proteins in polyacrylamide in a multiwell format
| Autor: | Edwin D. Cummings, Robert H. Waldo, George M. Hilliard, Siva T. Sarva, Janet M. Brown |
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| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Chromatography Peptide mapping Polyacrylamide Molecular Sequence Data Acrylic Resins High throughput proteomics A protein Proteins Serum Albumin Bovine General Chemistry Biology Biochemistry Peptide Mapping Silver stain chemistry.chemical_compound chemistry Animals Protein identification Cattle Trypsin Amino Acid Sequence |
| Zdroj: | Journal of proteome research. 6(4) |
| ISSN: | 1535-3893 |
| Popis: | Processing multiple protein samples from polyacrylamide at significant sensitivity represents a major chokepoint for raising the success rate in high-volume protein identification projects. A multiwell filterplate method for processing proteins in polyacrylamide was optimized for sensitivity using a protein standard. The results demonstrate this process to be a reliable and reproducible method over a range of gel loadings and suitable for the identification of proteins near the threshold of silver stain. This high-throughput manual method requires a minimum of specialized equipment, and can be performed disconnected from a proteomics infrastructure for the preparation of mass spectrometry-ready samples. |
| Databáze: | OpenAIRE |
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