How to develop an in-house real-time quantitative cytomegalovirus polymerase chain reaction: Insights from a cancer centre in Eastern India
| Autor: | Mammen Chandy, Anusha Harishankar, Sanjay Bhattacharya |
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| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Process management media_common.quotation_subject Congenital cytomegalovirus infection lcsh:QR1-502 Developing country Cytomegalovirus India standardisation Real-Time Polymerase Chain Reaction lcsh:Microbiology law.invention law Neoplasms Cancer centre medicine Humans Quality (business) Polymerase chain reaction media_common validation troubleshooting Health Care Costs medicine.disease Eastern india viral load Molecular Diagnostic Techniques Cytomegalovirus Infections Business |
| Zdroj: | Indian Journal of Medical Microbiology, Vol 33, Iss 4, Pp 482-490 (2015) |
| ISSN: | 1998-3646 |
| Popis: | Development of a reliable, cost-effective cytomegalovirus quantitative polymerase chain reaction (QPCR) is a priority for developing countries. Manufactured kits are expensive, and availability can be inconsistent. Development of an in-house QPCR kit that is reliable and quality assured requires significant effort and initial investment. However, the rewards of such an enterprise are manifold and include an in-depth understanding of molecular reactions, and expertise in the development of further low-cost molecular kits. The experience of an oncology centre in Eastern India has been shared. Hopefully, this would provide a brief roadmap for such an initiative. Staff with adequate understanding of molecular processes are essential along with vital infrastructure for molecular research and development. |
| Databáze: | OpenAIRE |
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