Protein A/G-based immunochromatographic test for serodiagnosis of pythiosis in human and animal subjects from Asia and Americas
| Autor: | Bunkuea Chantrathonkul, Janio Morais Santurio, Kavi Ratanabanangkoon, Yothin Kumsang, Amy M. Grooters, Papada Chaisuriya, Akarin Intaramat, Nujarin Jongruja, Theerapong Krajaejun, Tassanee Lohnoo, Wanta Yingyong, Alisa Sandee, Thiwaree Sornprachum, Ramrada Banyong, Angkana Chaiprasert |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Asia Thalassemia 030106 microbiology Blood Donors Enzyme-Linked Immunosorbent Assay Pythium Pythium insidiosum Sensitivity and Specificity Chromatography Affinity Serology 03 medical and health sciences Pythiosis Dogs parasitic diseases Protein A/G Medicine Animals Humans Serologic Tests Horses Pathogen Antibodies Fungal biology business.industry General Medicine biology.organism_classification medicine.disease Virology 030104 developmental biology Infectious Diseases Infectious disease (medical specialty) Immunoglobulin G biology.protein Cattle Rabbits Antibody Americas business |
| Zdroj: | Medical mycology. 54(6) |
| ISSN: | 1460-2709 |
| Popis: | Pythiosis is a life-threatening infectious disease of both humans and animals living in Asia, Americas, Africa, and parts of Australia and New Zealand. The etiologic pathogen is the fungus-like organism Pythium insidiosum The disease has high mortality and morbidity rates. Use of antifungal drugs are ineffective against P. insidiosum, leaving radical surgery the main treatment option. Prompt treatment leads to better prognosis of affected individuals, and could be achieved by early and accurate diagnosis. Since pythiosis has been increasingly reported worldwide, there is a need for a rapid, user-friendly, and efficient test that facilitates the diagnosis of the disease. This study aims to develop an immunochromatographic test (ICT), using the bacterial protein A/G, to detect anti-P. insidiosum IgGs in humans and animals, and compare its diagnostic performance with the established ELISA. Eighty-five serum samples from 28 patients, 24 dogs, 12 horses, 12 rabbits, and 9 cattle with pythiosis, and 143 serum samples from 80 human and 63 animal subjects in a healthy condition, with thalassemia, or with other fungal infections, were recruited for assay evaluation. Detection specificities of ELISA and ICT were 100.0%. While the detection sensitivity of ELISA was 98.8%, that of ICT was 90.6%. Most pythiosis sera, that were falsely read negative by ICT, were weakly positive by ELISA. In conclusion, a protein A/G-based ICT is a rapid, user-friendly, and efficient assay for serodiagnosis of pythiosis in humans and animals. Compared to ELISA, ICT has an equivalent detection specificity and a slightly lower detection sensitivity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|