Arabidopsis Qc-SNARE gene AtSFT12 is involved in salt and osmotic stress responses and Na(+) accumulation in vacuoles
| Autor: | Ji-Won Baik, Yong-Hwan Moon, Taijoon Chung, Sun-Young Lee, Dong-Hyuk Woo, Huong T. Tran, Dinh Huan Le, Vaishali N. Tarte, Hye-Yeon Seok, In Soon Kang |
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| Rok vydání: | 2014 |
| Předmět: |
Osmosis
Time Factors Osmotic shock Transcription Genetic Mutant Arabidopsis Golgi Apparatus Plant Science Vacuole Sodium Chloride Genes Plant symbols.namesake Gene Expression Regulation Plant Stress Physiological Organelle medicine Qc-SNARE Proteins biology Arabidopsis Proteins Sodium Gene Expression Regulation Developmental General Medicine Golgi apparatus Plant cell biology.organism_classification Plants Genetically Modified Cell biology Protein Transport Organ Specificity Mutation Vacuoles symbols Mannitol Agronomy and Crop Science medicine.drug Subcellular Fractions |
| Zdroj: | Plant cell reports. 34(7) |
| ISSN: | 1432-203X |
| Popis: | AtSFT12, an Arabidopsis Qc-SNARE protein, is localized to Golgi organelles and is involved in salt and osmotic stress responses via accumulation of Na + in vacuoles. To reduce the detrimental effects of environmental stresses, plants have evolved many defense mechanisms. Here, we identified an Arabidopsis Qc-SNARE gene, AtSFT12, involved in salt and osmotic stress responses using an activation-tagging method. Both activation-tagged plants and overexpressing transgenic plants (OXs) of the AtSFT12 gene were tolerant to high concentrations of NaCl, LiCl, and mannitol, whereas loss-of-function mutants were sensitive to NaCl, LiCl, and mannitol. AtSFT12 transcription increased under NaCl, ABA, cold, and mannitol stresses but not MV treatment. GFP-fusion AtSFT12 protein was juxtaposed with Golgi marker, implying that its function is associated with Golgi-mediated transport. Quantitative measurement of Na+ using induced coupled plasma atomic emission spectroscopy revealed that AtSFT12 OXs accumulated significantly more Na+ than WT plants. In addition, Na+-dependent fluorescence analysis of Sodium Green showed comparatively higher Na+ accumulation in vacuoles of AtSFT12 OX cells than in those of WT plant cells after salt treatments. Taken together, our findings suggest that AtSTF12, a Golgi Qc-SNARE protein, plays an important role in salt and osmotic stress responses and functions in the salt stress response via sequestration of Na+ in vacuoles. |
| Databáze: | OpenAIRE |
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