Ethanol Increases K+ Conductance in Human T-Cells
| Autor: | Louis J. DeFelice, Dorothy R. Oleson, Robert M. Donahoe |
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| Rok vydání: | 1993 |
| Předmět: |
Membrane potential
Potassium Channels Ethanol Chemistry T-Lymphocytes Pipette Medicine (miscellaneous) Conductance Hyperpolarization (biology) Lymphocyte Activation Toxicology Resting potential Potassium channel Membrane Potentials Psychiatry and Mental health Electrophysiology Biochemistry Immune Tolerance Potassium Biophysics Humans Reversal potential Cells Cultured |
| Zdroj: | Alcoholism: Clinical and Experimental Research. 17:604-609 |
| ISSN: | 1530-0277 0145-6008 |
| Popis: | Ethanol increased the open probability of a K channel in cultured human T-cells. Single-channel currents were studied using the patch-clamp technique in cell-attached configuration. Ethanol increased the number of simultaneously active channels and subsequent current maxima at concentrations of 35 and 50 mM from control levels of 3.2 to 4.6 pA and 8.4 pA, respectively. Current responses were elicited by a 80 mV hyperpolarizing pulse following adjustment to the same resting potential. The channel was K-selective as determined by the reversal potentials under different Na-K gradients. When the pipette contained 155 mM K, single-channel currents had a slope conductance of 28 pS and a reversal potential of -4.3 mV. Baseline current levels often shifted in a negative direction during the application of ethanol, indicating a hyperpolarization of the membrane potential and an increase in channel activity. |
| Databáze: | OpenAIRE |
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