Role of Zc3h12a in enhanced IL-6 production by newborn mononuclear cells in response to lipopolysaccharide
| Autor: | Tong-Xin Chen, Jian-Hua Sun, Jing Wu, Yi-Dan Zhang, Chen-xing Zhang, Xia-Fang Chen, Xu-Ting Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Lipopolysaccharides Lipopolysaccharide neonatal sepsis Peripheral blood mononuclear cell Procalcitonin Monocytes Pathogenesis 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ribonucleases 030225 pediatrics Medicine Humans Interleukin 6 Child IL-6 biology Neonatal sepsis business.industry Interleukin-6 lcsh:RJ1-570 Infant Newborn NF-kappa B Zc3h12a lcsh:Pediatrics Venous blood medicine.disease MyD88 Toll-Like Receptor 4 030104 developmental biology C-Reactive Protein chemistry Cord blood Child Preschool Pediatrics Perinatology and Child Health Immunology biology.protein business Transcription Factors |
| Zdroj: | Pediatrics and Neonatology, Vol 59, Iss 3, Pp 288-295 (2018) |
| ISSN: | 2212-1692 |
| Popis: | The uncontrolled inflammatory response following infection is closely related to the morbidity and mortality of neonates. Interleukin 6 (IL-6) plays an important role in the pathogenesis and prognosis of this process. To better elucidate the secretion of IL-6 following infection in neonates, we investigated the IL-6 level and mechanism of IL-6/TLR4 signaling pathways. Methods: We compared the IL-6, procalcitonin (PCT), and CRP levels between septic neonates and toddlers. In vitro cord blood samples from healthy term neonates and peripheral venous blood from healthy adult volunteers were collected. Protein expression was analyzed by Western blotting, mRNA expression by real-time PCR and membrane molecule expression by flow cytometry. Results: The IL-6 concentrations were significantly higher in the neonate group than in the toddler group (p |
| Databáze: | OpenAIRE |
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