SP-A Deficiency in Primate Model of Bronchopulmonary Dysplasia with Infertion: In Situ mRNA and Immunostains
Autor: | Robert A. deLemos, Steven R. Seidner, Jacqueline J. Coalson, Funmei Yang, Vicki T. Winter, Richard J. King, Jeffrey A. Whitsett |
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Rok vydání: | 1995 |
Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Pulmonary Surfactant-Associated Proteins Proteolipids medicine.medical_treatment Gene Expression In situ hybridization Critical Care and Intensive Care Medicine Positive-Pressure Respiration mental disorders medicine Animals Humans RNA Messenger Hysterotomy Lung Escherichia coli Infections In Situ Hybridization Bronchopulmonary Dysplasia Glycoproteins Pulmonary Surfactant-Associated Protein A business.industry Respiratory disease Infant Newborn Gestational age Pulmonary Surfactants medicine.disease Immunohistochemistry Pulmonary Alveoli Microscopy Electron medicine.anatomical_structure Animals Newborn Bronchopulmonary dysplasia Gestation business Immunostaining Papio Respiratory tract |
Zdroj: | American Journal of Respiratory and Critical Care Medicine. 151:854-866 |
ISSN: | 1535-4970 1073-449X |
Popis: | The surfactant protein secretory cells in airway and alveolar epithelium were studied in premature baboons with bronchopulmonary dysplasia and superimposed infection. PRN animals were delivered by hysterotomy at 140 d gestational age and ventilated on clinically appropriate oxygen for a 16-d experimental period. To assess 0 time and sacrifice time gestational parameters, 140 and 156 d were studied. BPD animals were delivered at 140 d and ventilated with positive-pressure ventilation and an FIO2 of 1.0 for 11 d followed by 5 d of oxygen sufficient to maintain PAO2 at 40 to 50 mm Hg. BPD-infected animals were comparably ventilated and treated like the BPD group except that 10(8) E. coli organisms were endotracheally instilled on Day 11. In situ hybridization studies for mRNA expression of SP-A, SP-B, and SP-C revealed that an SP-A mRNA deficiency, present at 140 d, persisted in the BPD and BPD-infected groups, whereas SP-A mRNA was abundant in PRN and 156 d gestation control groups. SP-B and SP-C mRNA expression in the two hyperoxically injured groups was particularly extensive in cells around peribronchiolar and perivasicular sites. Immunostaining with SP-A, SP-B, and SP-C antibodies showed variable staining patterns. The study clearly demonstrates that a deficiency of SP-A mRNA expression persists in chronic lung injury and that variable protein staining patterns are manifested depending upon the underlying pathology. |
Databáze: | OpenAIRE |
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