Nuclear Factor of Activated T Cells and Cytokines Gene Expression of the T Cells in AIDS Patients with Immune Reconstitution Inflammatory Syndrome during Highly Active Antiretroviral Therapy
| Autor: | Qihui Zhou, Ying Huang, Lijun Xu, Biao Zhu, Jia Sun, Junwei Su, Yirui Xie, Michael T. Yin, Heling Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine CD3 Complex Article Subject T-Lymphocytes Interleukin-1beta Immunology 03 medical and health sciences 0302 clinical medicine Immune reconstitution inflammatory syndrome Immune Reconstitution Inflammatory Syndrome Antiretroviral Therapy Highly Active Gene expression medicine lcsh:Pathology Humans 030212 general & internal medicine Interleukin 8 Acquired Immunodeficiency Syndrome NFATC Transcription Factors Interleukin-6 Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha business.industry Interleukin-8 Interleukin-18 NFAT Cell Biology medicine.disease Interleukin-10 Interleukin 10 030104 developmental biology Real-time polymerase chain reaction Cytokines Female Interleukin 18 Tumor necrosis factor alpha business Research Article lcsh:RB1-214 |
| Zdroj: | Mediators of Inflammation, Vol 2017 (2017) Mediators of Inflammation |
| ISSN: | 0962-9351 |
| DOI: | 10.1155/2017/1754741 |
| Popis: | Background. The etiology of immune reconstitution inflammatory syndrome (IRIS) in AIDS patients after the initiation of HAART remains unknown. Several researches indicated that the development of IRIS is associated with the production and variation of cytokines, whose gene expression are closely related to the Ca2+/CN-nuclear factor of activated T cells (NFAT) pathway.Methods. We studied the expression of NFAT isoforms and their major target cytokines genes in peripheral blood CD3+T cells of subjects through fluorescence quantitative PCR and explored the expression changes of these genes before and after HAART.Results. After the initiation of HARRT, NFAT1, IL-6, and IL-8 gene expression showed a reversal trend in the CD3+T cells of the IRIS group and changed from low expression before HARRT to high expression after HARRT. In particular, the relative gene expression of NFAT1 was markedly higher compared with the other three isoforms. The IRIS group also showed higher NFAT4, NFAT2, NFAT1, IL-1β, IL-10, IL-2, IL-18, and TNF-αgene expression than the non-IRIS group.Conclusion. This study suggested that high expression levels of IL-2, IL-6, IL-8, TNF-α, IL-1β, IL-10, IL-12, and IL-18 can predict the risk of IRIS. The increased expression of NFAT1 and NFAT4 may promote the expression of cytokines, such as IL-6, IL-8, and TNF-α, which may promote the occurrence of IRIS. |
| Databáze: | OpenAIRE |
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