Selection of Annonaceae Species for the Control of Spodoptera frugiperda (Lepidoptera: Noctuidae) and Metabolic Profiling of Duguetia lanceolata Using Nuclear Magnetic Resonance Spectroscopy
| Autor: | D. F. Oliveira, G. A. Carvalho, Alan Rodrigues Teixeira Machado, V. A. C. Campos, D. S. Alves |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Insecticides Magnetic Resonance Spectroscopy Metabolite Annonaceae Spodoptera 01 natural sciences Insect Control Duguetia Lepidoptera genitalia chemistry.chemical_compound Botany Toxicity Tests Animals Ecology biology General Medicine Nuclear magnetic resonance spectroscopy biology.organism_classification 010602 entomology chemistry Insect Science Proton NMR Noctuidae 010606 plant biology & botany |
| Zdroj: | Journal of economic entomology. 109(2) |
| ISSN: | 0022-0493 |
| Popis: | This study was performed to investigate the activity of 19 dichloromethane-soluble fractions obtained from the methanolic extracts of 10 Annonaceae species against the fall armyworm, Spodoptera frugiperda (J. E. Smith). The stem bark of Duguetia lanceolata A. St.-Hil. showed the highest insecticidal activity, with a median lethal time (LT50) of 61.4 h and a median lethal concentration (LC50) of 946.5 µg/ml of diet. The dichloromethane-soluble fractions from six D. lanceolata specimens were subjected to evaluation of their activities against S. frugiperda and metabolomic analysis using hydrogen (1H) nuclear magnetic resonance (NMR) spectroscopy. Although all of the samples affected S. frugiperda mortality, their insecticidal activities varied according to the sample used in the experiments. Using partial least squares regression of the results, the D. lanceolata specimens were grouped according to their metabolite profile and insecticidal activity. A detailed analysis via uni- and bidimensional NMR spectroscopy showed that the peaks in the 1H NMR spectra associated with increased insecticidal activity could be attributed to 2,4,5-trimethoxystyrene, which suggests that this substance is involved in the insecticidal activity of the stem bark fraction of D. lanceolata . |
| Databáze: | OpenAIRE |
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